Human TGF-alpha ELISA

Old price: $402.00
$361.80
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RayBio® Human TGF alpha ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELH-TGFa-1
+

Antigen Information

Accession Number:
  • P01135
Gene ID:
  • 7039
Gene Symbol:
  • TGFA
Protein Name & Synonyms:
Protransforming growth factor alpha [Cleaved into: Transforming growth factor alpha (TGF-alpha) (EGF-like TGF) (ETGF) (TGF type 1)]
Target Species:
  • Human

Assay Format

Detection Range:
3 pg/ml - 1000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
3 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ALCAM, Angiopoietin-1, Angiostatin, B7-1, BMP-7, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4 , DR6, Endostatin, E-Selectin, HB-EGF, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-13 Ra1, IL-18 BPa, IL-18 Rb, IL-18, IL-2 Ra, MMP-1, IL-2 Rb, IL-2 Rg, IL-21 R, MMP-2 , MMP-3, MMP-10, MMP-13).
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Lim Y., Yoo J., Kim M., et al. GC1118, an Anti-EGFR Antibody with a Distinct Binding Epitope and Superior Inhibitory Activity against High-Affinity EGFR Ligands. Mol Cancer Ther. 2016 Feb;15(2):251-63. doi: 10.1158/1535-7163.MCT-15-0679
    Species: Human
    Sample type: Conditioned Media
  2. Ekblad L., et al. Anti- or pro-proliferation - Conditional options for TGF-a and cetuximab in head and neck squamous cell carcinoma
    Species: Human
    Sample type: Conditioned Media
  3. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample type: Tissue Lysate
  4. Miyabayashi K, Ijichi H, Mohri D, et al. Erlotinib prolongs survival in pancreatic cancer by blocking gemcitabine-induced MAPK signals. Cancer Res. 2013 Apr 1;73(7):2221-2234.
    Species: Human
    Sample type:
  5. Koob, Thomas J., et al. "Biological properties of dehydrated human amnion/chorion composite graft: implications for chronic wound healing." International wound journal 10.5 (2013): 493-500.
    Species: Human
    Sample type: Tissue Lysate
  6. Rayego?Mateos, Sandra, et al. "TWEAK transactivation of the epidermal growth factor receptor mediates renal inflammation." The Journal of pathology 231.4 (2013): 480-494.
    Species: Human
    Sample type: Conditioned Media
  7. Franzke C-W, Cobzaru C, Triantafyllopoulou A, et al. Epidermal ADAM17 maintains the skin barrier by regulating EGFR ligand-dependent terminal keratinocyte differentiation. The Journal of Experimental Medicine 2012;209(6):1105-1119. doi:10.1084/jem.20112258.
    Species: Human
    Sample type: Conditioned Media
  8. Briana D., Liosi S., Gourgiotis D., et al. Fetal concentrations of the growth factors TGF-? and TGF-?1 in relation to normal and restricted fetal growth at term. Cytokine. 2012 Oct;60(1):157-61. doi: 10.1016/j.cyto.2012.06.005
    Species: Human
    Sample type: Plasma
  9. Pinto, C., et al. "Phase II study of panitumumab, oxaliplatin, 5-fluorouracil, and concurrent radiotherapy as preoperative treatment in high-risk locally advanced rectal cancer patients (StarPan/STAR-02 Study)." Annals of oncology 22.11 (2011): 2424-2430.
    Species: Human
    Sample type: Serum
  10. Lig?za, Janusz, Joanna Lig?za, and Andrzej Klein. "Growth factor/growth factor receptor loops in autocrine growth regulation of human prostate cancer DU145 cells." Acta Biochim Pol 58.3 (2011): 391-396.
    Species: Human
    Sample type: Conditioned Media
The human TGF-alpha ELISA kit has a direct and very simple instructions that is easy to follow and work with. We used the kit with TGF-alpha-secreting human cell lines following the manufacturer's protocol. Cells were treated with an inhibitor to detect the decrease in TGF-alpha secretion. Also, supernatant was used to detect the TGF-alpha content. The kit worked perfectly, however, my concern is very minor but I think its important to raise, the wash buffer have visible crystals that even after you thawed in 37C or RT for quite some time, the crystals are still there. I am just concern that this crystals will affect the washing step and will further affect the result. Overall, the kit works well in detecting TGF-alpha and we are pleased with the results.
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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