Human TGF-beta 1 ELISA

RayBio® Human TGF beta 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-TGFb1-1
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Antigen Information

Accession Number:
  • P01137
Gene ID:
  • 7040
Gene Symbols:
  • TGFB
  • TGFB1
Protein Name & Synonyms:
Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)]
Target Species:
  • Human

Assay Format

Detection Range:
18 pg/ml - 4000 pg/ml
Recommended Dilution (serum & plasma):
20 fold after treatment (see activation steps on page 6)
Sensitivity:
18 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Xing W., Xiao Y., Lu., et al. GFI1 downregulation promotes inflammation-linked metastasis of colorectal cancer. Cell Death Differ. 2017 May; 24(5): 929–943.
    Species: Human
    Sample type: Conditioned Media
  2. Diaz-Gomez L., Concheiro A., Alvarez-Lorenzo C., Garcia-Gonzalez C. Growth factors delivery from hybrid PCL-starch scaffolds processed using supercritical fluid technology. Carbohydr Polym. 2016 May 20;142:282-92. doi: 10.1016/j.carbpol.2016.01.051.
    Species: Human
    Sample type: Conditioned Media
  3. Sun Y., Peng X., Zhang H., et al. P53 is required for Doxorubicin-induced apoptosis via the TGF-beta signaling pathway in osteosarcoma-derived cells. Am J Cancer Res. 2016; 6(1): 114-125.
    Species: Human
    Sample type: Conditioned Media
  4. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF?7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016
    Species: Human
    Sample type: Conditioned Media
  5. Jah A., Tharp K., Ye J., et al. Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels. Biomaterials. 2015 Apr;47:1-12. doi: 10.1016/j.biomaterials.2014.12.043.
    Species: Human
    Sample type: Conditioned Media
  6. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample type: Conditioned Media
  7. Shen C., Lie P., Miao T., et al. Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis. Mol Med Rep. 2015 Jul;12(1):20-30. doi: 10.3892/mmr.2015.3409
    Species: Human
    Sample type: Conditioned Media
  8. Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-?, IL-1? and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718
    Species: Rat
    Sample type: Tissue Lysate
  9. Tang J., Chen Q., Guo J., et al. Minocycline Attenuates Neonatal Germinal-Matrix-Hemorrhage-Induced Neuroinflammation and Brain Edema by Activating Cannabinoid Receptor 2. Mol Neurobiol. 2015 Apr 2. epub ahead of print
    Species: Rat
    Sample type: Tissue Lysate
  10. Jha A., Mathur A., Svedlund F., et al. Molecular weight and concentration of heparin in hyaluronic acid-based matrices modulates growth factor retention kinetics and stem cell fate. J Control Release. 2015 Jul 10;209:308-16. doi: 10.1016/j.jconrel.2015.04.034.
    Species: Human
    Sample type: Conditioned Media
Correlates with Kiloplex results

University of Houston
Useful in measuring hTGFB1 concentration for nanoparticle incorporation of growth factors. ELISA kit instructions were easy to follow and had a high sensitivity which is necessary in specific essays like this.

University of Wisconsin-Madison
I used it for human corneal epithelial cell culture supernatant under induced conditions and tested various treatments. It is very important to ensure that it's very high sensitivity and this product was very useful.

University of Illinois at Chicago
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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