Human TGF-beta 1 ELISA

$398.00
ELH-TGFb1-1

RayBio® Human TGF beta 1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
80 pg/ml

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Detection Range
80 pg/ml - 60 ng/ml
Recommended Dilution (serum & plasma)
3 fold after treatment (see activation steps on page 6)
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7
Species Detected
Human
Protein Name & Synonyms
Transforming growth factor beta-1 (TGF-beta-1)
Accession Number
Gene Names
TGFB1, TGFB
Gene ID
7040

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • TGFB1

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Diaz-Gomez L., Concheiro A., Alvarez-Lorenzo C., Garcia-Gonzalez C. Growth factors delivery from hybrid PCL-starch scaffolds processed using supercritical fluid technology. Carbohydr Polym. 2016 May 20;142:282-92. doi: 10.1016/j.carbpol.2016.01.051.
    Species: Human
    Sample Type: Conditioned Media

  2. Sun Y., Peng X., Zhang H., et al. P53 is required for Doxorubicin-induced apoptosis via the TGF-beta signaling pathway in osteosarcoma-derived cells. Am J Cancer Res. 2016; 6(1): 114-125.

  3. Kohh B., Nadarajan K., Shim S., et al. Pretreatment of BMSCs with TZD solution decreases the proliferation rate of MCF‑7 cells by reducing FGF4 protein expression. Molecular Medicine Reports 13: 3406-3414, 2016

  4. Jah A., Tharp K., Ye J., et al. Enhanced survival and engraftment of transplanted stem cells using growth factor sequestering hydrogels. Biomaterials. 2015 Apr;47:1-12. doi: 10.1016/j.biomaterials.2014.12.043.
    Species: Human
    Sample Type: Conditioned Media

  5. Shen C., Lin L., Feng T., et al. Dental Pulp Stem Cells derived Conditioned Medium Promotes Angiogenesis in Hindlimb Ischemia. DOI:10.1007/s13770-014-9053-7Tissue Engineering and Regenerative Medicine February 2015, Volume 12, Issue 1, pp 59-68 .
    Species: Human
    Sample Type: Conditioned Media

  6. Shen C., Lie P., Miao T., et al. Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis. Mol Med Rep. 2015 Jul;12(1):20-30. doi: 10.3892/mmr.2015.3409
    Species: Human
    Sample Type: Conditioned Media

  7. Chen H., Min XH., Wang QY., et al. Pre-activation of mesenchymal stem cells with TNF-α, IL-1β and nitric oxide enhances its paracrine effects on radiation-induced intestinal injury. Scientific Reports 5, Article number: 8718 (2015). doi:10.1038/srep08718
    Species: Rat
    Sample Type: Tissue Lysate

  8. Tang J., Chen Q., Guo J., et al. Minocycline Attenuates Neonatal Germinal-Matrix-Hemorrhage-Induced Neuroinflammation and Brain Edema by Activating Cannabinoid Receptor 2. Mol Neurobiol. 2015 Apr 2. epub ahead of print
    Species: Rat
    Sample Type: Tissue Lysate

  9. Jha A., Mathur A., Svedlund F., et al. Molecular weight and concentration of heparin in hyaluronic acid-based matrices modulates growth factor retention kinetics and stem cell fate. J Control Release. 2015 Jul 10;209:308-16. doi: 10.1016/j.jconrel.2015.04.034.
    Species: Human
    Sample Type: Conditioned Media

  10. El-Haggar Sm., Mostafa TM. Comparative clinical study between the effect of fenofibrate alone and its combination with pentoxifylline on biochemical parameters and liver stiffness in patients with non-alcoholic fatty liver disease. Hepatol Int. 2015 Jul;9(3):471-9. doi: 10.1007/s12072-015-9633-1.
    Species: Human
    Sample Type: Plasma

  11. Sowmya S., Chennazhi K., Arzate H., et al. Periodontal Specific Differentiation of Dental Follicle Stem Cells into Osteoblast, Fibroblast, and Cementoblast. Tissue Eng Part C Methods. 2015 Oct;21(10):1044-58. doi: 10.1089/ten.TEC.2014.0603.
    Species: Human
    Sample Type: Conditioned Media

  12. Yi H., Yu C., Zhang H., et al. Cathelicidin-BF suppresses intestinal inflammation by inhibiting the nuclear factor-κ signaling pathway and enhancing the phagocytosis of immune cells via STAT-1 in weanling piglets. Int Immunopharmacol. 2015 Sep;28(1):61-9. doi: 10.1016/j.intimp.2015.05.034
    Species: Porcine
    Sample Type: Serum

  13. Tran, H.L.B., Nguyen, M.T.N. & Doan, V.N. Fabrication and evaluation of human dentin as scaffold for dental pulp stem cells. Tissue Eng Regen Med (2015) 12: 222. doi:10.1007/s13770-014-0103-y
    Species: Human
    Sample Type: Conditioned Media

  14. Attia EAS., et al. Serum Th17 cytokines in leprosy: correlation with circulatingCD4+ CD25highFoxP3+ T-regs cells, as well as down regulatory cytokines. Arch Dermatol Res. Published online July 2014. DOI 10.1007/s00403-014-1486-2
    Species: Human
    Sample Type: Serum

  15. Chen X., et al. Urinary TGF-1 has a supplementary value in predicting renalfunction recovery post unilateral ureteral obstruction. Int Urol Nephrol., October 2014 epub ahead of print. DOI 10.1007/s11255-014-0846-3
    Species: Human
    Sample Type: Urine

  16. Chowdhurya I.H., et al. Alteration of serum inflammatory cytokines in active pulmonarytuberculosis following anti-tuberculosis drug therapy. Molecular Immunology 62 (2014) 159-168
    Species: Human
    Sample Type: Serum

  17. Gao, S., Dine, J., et al. Anti-inflammatory and Anti-apoptotic Effect of Combined Treatment with Methylprednisolone and Amniotic Membrane Mesenchymal Stem Cells After Spinal Cord Injury in Rats. Neurochemical Research 2014. Epub Ahead of Print DOI:10.1007/s11064-014-1344-9
    Species: Human
    Sample Type: Tissue Lysate

  18. Gravina et al.: XPO1/CRM1-Selective Inhibitors ofNuclear Export (SINE) reduce tumor spreading and improve overallsurvival in preclinical models of prostate cancer (PCa). Journal ofHematology & Oncology 2014 7:46.
    Species: Human
    Sample Type: Conditioned Media

  19. Jun EK, Zhang Q, Yoon BS, et al. Hypoxic Conditioned Medium from Human Amniotic Fluid-Derived Mesenchymal Stem Cells Accelerates Skin Wound Healing through TGF-β/SMAD2 and PI3K/Akt Pathways. International Journal of Molecular Sciences 2014;15(1):605-628. doi:10.3390/ijms15010605.
    Species: Human
    Sample Type: Conditioned Media

  20. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample Type: Tissue Lysate

  21. Koob TJ., Lim JJ., Massee M., Zabek N., Denoziere G. Properties of dehydrated human amnion/chorion composite grafts: Implications for wound healing and soft tissue regenereation. J Biomed Mater Res B Appl Biomater. 2014 Mar 25 [Epub ahead of print]
    Species: Human
    Sample Type: Tissue Lysate

  22. Gravina GL, Mancini A, Ranieri G, et al. Phenotypic characterization of human prostatic stromal cells in primary cultures derived from human tissue samples. Int J Oncol. 2013 Jun;42(6):2116-2122.
    Species: Human
    Sample Type: Conditioned Media

  23. Kanlaya R, Sintiprungrat K, Thongboonkerd V. Secreted Products of Macrophages Exposed to Calcium Oxalate Crystals Induce Epithelial Mesenchymal Transition of Renal Tubular Cells via RhoA-Dependent TGF-β1 Pathway. Cell Biochem. Biophys. 2013 May 23; [Epub ahead of print] doi:10.1007/s12013-013-9639-z
    Species: Human
    Sample Type: Conditioned Media

  24. Zou W, Zou Y, Zhao Z, et al. Nicotine-induced epithelial-mesenchymal transition via Wnt/β-catenin signaling in human airway epithelial cells. Am. J. Physiol. Lung Cell Mol. Physiol. 2013 Feb 15;304(4):L199-209.
    Species: Human
    Sample Type: Conditioned Media

  25. Fullár A, Kovalszky I, Bitsche M, et al. Tumor cell and carcinoma-associated fibroblast interaction regulates matrix metalloproteinases and their inhibitors in oral squamous cell carcinoma. Experimental Cell Research. 2012;318(13-16):1517-1527. doi:10.1016/j.yexcr.2012.03.023.
    Species: Human
    Sample Type: Conditioned Media

  26. Lohmann M, Walenda G, Hemeda H, et al. Donor Age of Human Platelet Lysate Affects Proliferation and Differentiation of Mesenchymal Stem Cells. Prosper F, ed. PLoS ONE. 2012;7(5):e37839. doi:10.1371/journal.pone.0037839.
    Species: Human
    Sample Type: Platelet Releasate

  27. Dragoo J., Korotkova T., Wasterlain A., et al. Age-Related Changes of Chondrogenic Growth Factors in Platelet-Rich Plasma. Operative Techniques in Orthopaedics, Volume 22, Issue 2, June 2012, Pages 49-55
    Species: Human
    Sample Type: Plasma

  28. Nokhbehsaim, Marjan, et al. "Effects of enamel matrix derivative on periodontal wound healing in an inflammatory environment in vitro." Journal of Clinical Periodontology 38.5 (2011): 479-490.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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