Human Thrombospondin-5/COMP ELISA Kit

RayBio® Human Thrombospondin-5/COMP ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Human Thrombospondin-5/COMP ELISA Kit

RayBio® Human Thrombospondin-5/COMP ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Sensitivity	0.085 ng/ml Need more sensitivity? Check out the new NexaTag^® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa^™ technology.
Detection Range	0.085 ng/ml - 20 ng/ml
Specificity	This ELISA kit shows no cross-reactivity with the following cytokines tested: human ADAMTS-13, Aggrecan, Angiotensinogen (SERPINA8), B7- H1 (CD274), BMPR-IA (ALK-3), BMPR-II, Cadherin-11, CD27 (TNFRSF7), CD6, Ck beta 8-1 (CCL23), CNTF, DNAM1 (CD226), CD147 (EMMPRIN), FLRG, Follistatin-like 1, Fractalkine (CX3CL1), Galectin-1, GITR Ligand (TNFSF18), Granulysin (NKG5/LAG-2), IL-1 R3 (IL-1 R Acp), IL-15 R alpha, IL-17E (IL-25), IL-32 alpha, CHL-1 (L1CAM-2), LRIG3, LRP-6, MEPE (OF45), Nectin-4, Periostin, Persephin, Renin, RGM-B, ROBO3, S100 A8, Siglec-7 (CD328), Syndecan-3, Thrombospondin 1, Thrombospondin 2, Thrombospondin 3, Thrombospondin 4, Tie-1, ULBP-2 (ALCAN-alpha/N2DL2).
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)	150 fold
Accession Number	P49747
Gene Id	1311
Gene Symbols	COMP
Protein Name / Synonyms	Cartilage oligomeric matrix protein (COMP) (Thrombospondin-5) (TSP5)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Shipping Type	Blue ice
Storage	≤-20°C

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4^°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

May be stored for up to 6 months at 2^° to 8^°C from the date of shipment. Standard (recombinant protein) should be stored at -20^°C or -80^°C (recommended at -80^°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2^° to 8^°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20^°C.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (ELH-TSP5.pdf)

SDS (ELH-TSP5.pdf)

Excel (5af824478c7690acd2cf4518aeaae2058a44fa75.xlsm)

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4^°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

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Documents

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