Human TIMP-1 ELISA

$398.00
ELH-TIMP1-1

RayBio® Human TIMP-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
40 pg/ml

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Detection Range
2 pg/ml - 1200 pg/ml
Recommended Dilution (serum & plasma)
5 - 100 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1 alpha, MIP-1 beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1 alpha, TNFbeta, TGFbeta1, TGFbeta2, TGFbeta3, VEGF.
Species Detected
Human
Protein Name & Synonyms
Metalloproteinase inhibitor 1 (Erythroid-potentiating activity) (EPA) (Fibroblast collagenase inhibitor) (Collagenase inhibitor) (Tissue inhibitor of metalloproteinases 1) (TIMP-1)
Accession Number
Gene Names
TIMP1, CLGI, TIMP
Gene ID
7076

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • TIMP1

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Prato M., Khadjavi A., Magnetto C., et al. Effects of oxygen tension and dextran-shelled/2H,3H-decafluoropentane-cored oxygen-loaded nanodroplets on secretion of gelatinases and their inhibitors in term human placenta. Biosci Biotechnol Biochem. 2016;80(3):466-72. doi: 10.1080/09168451.2015.1095068
    Species: Human
    Sample Type: Conditioned Media

  2. Hanedan O., Baykan A., Sezer S., et al. Evaluation of Cardiovascular Changes in Children with BAVs. Pediatr Cardiol. 2016 Mar;37(3):472-81. doi: 10.1007/s00246-015-1302-6.

  3. Humbert P., Fanian F., Lihoreau T., et al. Mécano-Stimulation™ of the skin improves sagging score and induces beneficial functional modification of the fibroblasts: clinical, biological, and histological evaluations. Clin Interv Aging. 2015 Feb 2;10:387-403. doi: 10.2147/CIA.S69752
    Species: Human
    Sample Type: Conditioned Media

  4. Gulino G., Magnetto C., Khadjavi A., et al. Oxygen-Loaded Nanodroplets Effectively Abrogate Hypoxia Dysregulating Effects on Secretion of MMP-9 and TIMP-1 by Human Monocytes. Mediators Inflamm. 2015;2015:964838. doi: 10.1155/2015/964838.
    Species: Human
    Sample Type: Conditioned Media

  5. Garratt L., Sutanto E., Ling K., et al. Matrix metalloproteinase activation by free neutrophil elastase contributes to bronchiectasis progression in early cystic fibrosis. Eur Respir J. 2015 Aug;46(2):384-94. doi: 10.1183/09031936.00212114.
    Species: Human
    Sample Type: BAL Fluid

  6. Khadjavi A., Magnetto C., Panariti A., et al. Chitosan-shelled oxygen-loaded nanodroplets abrogate hypoxia dysregulation of human keratinocyte gelatinases and inhibitors: New insights for chronic wound healing. Toxicol Appl Pharmacol. 2015 Aug 1;286(3):198-206. doi: 10.1016/j.taap.2015.04.015
    Species: Human
    Sample Type: Conditioned Media

  7. Markiewicz L., Pytel D., Mucha B., Szymanek K., et al. Altered Expression Levels of MMP1, MMP9, MMP12, TIMP1, and IL-1β as a Risk Factor for the Elevated IOP and Optic Nerve Head Damage in the Primary Open-Angle Glaucoma Patients. BioMed Research International Volume 2015 (2015), Article ID 812503, 8 pages http://dx.doi.org/10.1155/2015/812503
    Species: Human
    Sample Type: Aqueous Humor

  8. Ciaffi L., Cavassini M., Genne D., et al. Switch to etravirine for HIV-positive patients receiving statin treatment: a prospective study. Eur J Clin Invest. 2015 Jul;45(7):720-30. doi: 10.1111/eci.12464.
    Species: Human
    Sample Type: Serum

  9. Guan W., Gao Y., Xu G., et al. Sputum matrix metalloproteinase-8 and -9 and tissue inhibitor of metalloproteinase-1 in bronchiectasis: Clinical correlates and prognostic implications. Respirology (2015) 20, 1073-1081 doi: 10.1111/resp.12582
    Species: Human
    Sample Type: Sputum

  10. Wadood S., Shawk R., Hashem R. Variants of MMP-9 and TIMP-1 Levels Could be a Predictor of an Early Development of Cardiovascular Diseases in Type 2 Diabetes among Iraqi Patients. Iraqi Journal of Science, 2015, Vol 56, No.1C, pp:622-632
    Species: Human
    Sample Type: Serum

  11. Wang W., Song X., Chen Y., et al. The Long-Term Influence of Tissue Inhibitor of Matrix Metalloproteinase-1 in Patients with Mild to Moderate Coronary Artery Lesions in a Chinese Population: A 7-Year Follow-Up Study. Cardiology. 2015;132(3):151-8. doi: 10.1159/000430885
    Species: Human
    Sample Type: Plasma

  12. Basilico N., Magnetto C., D'Alessandro D., et al. Dextran-shelled oxygen-loaded nanodroplets reestablish a normoxia-like pro-angiogenic phenotype and behavior in hypoxic human dermal microvascular endothelium. Toxicol Appl Pharmacol. 2015 Nov 1;288(3):330-8. doi: 10.1016/j.taap.2015.08.005.
    Species: Human
    Sample Type: Conditioned Media

  13. Acar B., Nese, Z., et al. Serum MMP-2, MMP-9, TIMP-1 and TIMP-2 levels in multiple sclerosis clinical subtypes and their diagnostic value in the progressive disease course. Biomedical Research, Volume 25, Issue 3, Pages 343 - 350. 2014.
    Species: Human
    Sample Type: Serum

  14. Esa S., et al. Study of the level of sputum matrix metalloproteinase-9 (MMP-9) and tissue inhibitor metalloproteinase-1 (TIMP-1) in COPD patients. Egyptian Journal of Chest Diseases and Tuberculosis Available online 18 July 2014 DOI: 10.1016/j.ejcdt.2014.06.014
    Species: Human
    Sample Type: Sputum

  15. Fernandez-Yunquera A., et al. Everolimus immunosuppression reduces the serum expression of fibrosis markers in liver transplant recipients. World J Transplant 2014; 4(2): 133-140. http://dx.doi.org/10.5500/wjt.v4.i2.133
    Species: Human
    Sample Type: Serum

  16. Ghodpage P., et al. Influence of phase I periodontal therapy on levels of matrix metalloproteinase 1 and tissue inhibitor of metalloproteinase 1 . The Saudi Dental Journal. Available online 15 July 2014 DOI: 10.1016/j.sdentj.2014.05.003
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  17. Gluba-Brzozka A., et al. Markers of increased cardiovascular risk in patients with chronic kidney disease. Lipids in Health and Disease 2014, 13:135 doi:10.1186/1476-511X-13-135
    Species: Human
    Sample Type: Serum

  18. Kılıç Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anadolu Kardiyol Derg 2014; 14: 531-41. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample Type: Serum

  19. Kilic Z., et al. Circulating matrix metalloproteinases and tissue inhibitors of metalloproteinases levels in pediatric patients with congenital heart disease: Relationship to cardiac functions. Anatolian J of Card. Published ahead of print, June 25th 2014. DOI:10.5152/akd.2014.4950
    Species: Human
    Sample Type: Serum

  20. Koob T., et al. Cytokines in single layer amnion allografts compared to multilayeramnion/chorion allografts for wound healing. Journal of Biomedical Materials Research Part B: Applied Biomaterials. Aug 2014, epub ahead of print. DOI: 10.1002/jbm.b.33265
    Species: Human
    Sample Type: Tissue Lysate

  21. Popat R., Bhavsar NV., Popat PR. Gingival crevicular fluid levels of Matrix Metalloproteinase-1 (MMP-1) and Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) in periodontal health and disease. Singapore Dent J. 2014 Dec;35:59-64. doi: 10.1016/j.sdj.2014.07.003.
    Species: Human
    Sample Type: Gingival Crevicular Fluid

  22. Rolin G., et al. In vitro study of the impact of mechanical tension on the dermal fibroblast henotype in the context of skin wound healing. Journal of Biomechanics Sept 2014, epub ahead of print. http://dx.doi.org/10.1016/j.jbiomech.2014.07.015
    Species: Human
    Sample Type: Conditioned Media

  23. Tian F., Gu C., Zhao Z., et al. Urinary Emmprin, MMP-9 and TIMP-1 as potential biomarkers in children with ureteropelvic junction narrowing on conservative treatment. Nephrology (Carlton). 2014 Dec 3. doi: 10.1111/nep.12371
    Species: Human
    Sample Type: Urine

  24. Botta GP, Reichert M, Reginato MJ, et al. ERK2-regulated TIMP1 induces hyperproliferation of K-Ras(G12D)-transformed pancreatic ductal cells. Neoplasia. 2013;15(4):359-372.
    Species: Human
    Sample Type: Conditioned Media

  25. Hampel U, Klonisch T, Sel S, et al. Insulin-like Factor 3 Promotes Wound Healing at the Ocular Surface. Endocrinology. 2013;154(6):2034-2045.
    Species: Human
    Sample Type: Conditioned Media

  26. Mamlouk, O. et al. Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes. Int J Radiat Biol. 2012 Dec;88(12):1019-27. doi: 10.3109/09553002.2012.702297. Epub 2012 Jul 11
    Species: Human
    Sample Type: Conditioned Media

  27. Yilmax A., Bilge I., Kiyak A., Gedikbasi A., et al. Matrix metalloproteinase 9 and tissue inhibitor of metalloproteinase 1 in vesicoureteral reflux. Pediatr Nephrol. 2012 Mar;27(3):435-41. doi: 10.1007/s00467-011-2026-3.
    Species: Human
    Sample Type: Urine

  28. Raghu H., Nalla A., Gondi C., et al. uPA and uPAR shRNA inhibit angiogenesis via enhanced secretion of SVEGFR1 independent of GM-CSF but dependent on TIMP-1 in endothelial and glioblastoma cells. Mol Oncol. 2012 Feb;6(1):33-47. doi: 10.1016/j.molonc.2011.11.008.
    Species: Human
    Sample Type: Conditioned Media

  29. Yigit, Özgür, et al. "The effect of corticosteroid on metalloproteinase levels of nasal polyposis 1." The Laryngoscope 121.3 (2011): 667-673.

  30. Yigit, Özgür, et al. "The effect of corticosteroid on metalloproteinase levels of nasal polyposis 1." The Laryngoscope 121.3 (2011): 667-673.

  31. Upadhyay A, Sharma G, Kivivuori S, Raye W S, Zabihi E, et al. Role of a LIF antagonist in LIF and OSM induced MMP-1, MMP-3, and TIMP-1 expression by primary articular chondrocytes. Cytokine. 2009;46:332-338.
    Species: Porcine
    Sample Type: Conditioned Media

  32. Steed D, Trumpower C, Duffy D, Smith C, Marshall V, et al. Amnion-derived Cellular Cytokine Solution A Physiological Combination of Cytokines for Wound Healing. Eplasty. 2008;8:e18.
    Species: Human
    Sample Type: Conditioned Media

  33. Kralisch S, Lossner U, Bluher M, Paschke R, Stumvoll M, Fasshauer M. Tissue inhibitor of metalloproteinase 1 expression and secretion are induced by beta-adrenergic stimulation in 3T3-L1 adipocytes. J Endocrinol. 2006; 189:665-670.
    Species: Human
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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