Human VCAM-1 ELISA

RayBio® Human VCAM-1 (CD106) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Old price: $402.00
$321.60
You save: $80.40 (20%)
ELH-VCAM1-1
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Antigen Information

Accession Number:
  • P19320
Gene ID:
  • 7412
Gene Symbols:
  • L1CAM
  • VCAM1
Protein Name & Synonyms:
Vascular cell adhesion protein 1 (V-CAM 1) (VCAM-1) (INCAM-100) (CD antigen CD106)
Target Species:
  • Human

Assay Format

Detection Range:
0.3 ng/ml - 60 ng/ml
Recommended Dilution (serum & plasma):
10 - 50 fold
Sensitivity:
300 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiopoietin-1, Angiostatin , B7-1, BMP-7, CD14, CD30, CD40, CD40 Ligand, CTLA-4, CXCL16, Dkk-4, DR6, Endostatin, E-Selectin, Follistatin, HB-EGF, HVEM, ICAM-2, IGF-II, IL-10 Ra, IL-10 Rb, IL-18, IL-9 , IL-2 Ra, IL-2 Rb, IL-5 Ra, LAP, L-Selectin, MCSF R, MMP-1, 2, 3, 7, 8, 9, 10,12 and 13, PDGF-AB, SDF-1b, Tie-1, Tie- 2, TIMP-3.
Number of Targets Detected:
1
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Fadel F., Elshamaa M., Nabhan M., Essam R., Kantoush N., et al. Soluble adhesion molecules as markers of nativearteriovenous fistula thrombosis in children on uremia. Blood Coagulation and Firbrinolysis. Published online DOI:10.1097/MBC.0000000000000125 [Epub ahead of Print]
    Species: Human
    Sample type: Serum
  2. Wadood S., Shawk R., Baher H. Intercellular Adhesion Molecule-1, Vascular Adhesion Molecule-1, Interlukin1-?, and C - reactive protein Levels in Iraqi Patients with Type 2 Diabetes Mellitus. IOSR J of Dental and Medical SciencesVolume 13, Issue 4 Ver. IV. (Apr. 2014), PP55-62
    Species: Human
    Sample type: Serum
  3. Comparison of gene expression profile of epiretinal membranes obtained from eyes with proliferative vitreoretinopathy to that of secondary epiretinal membranes
    Species: Human
    Sample type: Vitreous Fluid
  4. Schlatzer DM, Sugalski JM, Chen Y, et al. Plasma proteome analysis reveals overlapping, yet distinct mechanisms of immune activation in chronic HCV and HIV infections. J Acquir Immune Defic Syndr. 2013 Mar 15; [Epub ahead of print] doi:10.1097/QAI.0b013e3182909847.
    Species: Human
    Sample type: Plasma
  5. Ro, Han, et al. "Roles of islet Toll-like receptors in pig to mouse islet xenotransplantation." Cell transplantation 22.9 (2013): 1709-1722.
    Species: Porcine
    Sample type: Conditioned Media
  6. Liu, Zhao-Min, et al. "Effect of soy protein and isoflavones on blood pressure and endothelial cytokines: a 6-month randomized controlled trial among postmenopausal women." Journal of hypertension 31.2 (2013): 384-392.
    Species: Human
    Sample type: Serum
  7. Chao, Pi-Yu, Ya-Ping Huang, and Wen-Bin Hsieh. "Inhibitive effect of purple sweet potato leaf extract and its components on cell adhesion and inflammatory response in human aortic endothelial cells." Cell adhesion & migration 7.2 (2013): 237-245.
    Species: Human
    Sample type: Conditioned Media
  8. Sadik N., Mohamed W., Ahmed M. The association of receptor of advanced glycated end products and inflammatory mediators contributes to endothelial dysfunction in a prospective study of acute kidney injury patients with sepsis. Mol Cell Biochem. 2012 Jan;359(1-2):73-81. doi: 10.1007/s11010-011-1001-4.
    Species: Human
    Sample type: Serum
  9. Tammali, Ravinder, et al. "Inhibition of aldose reductase prevents angiogenesis in vitro and in vivo." Angiogenesis 14.2 (2011): 209-221.
    Species: Human
    Sample type: Conditioned Media
Very sensitive assay system at great price. Highly recommended.

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Simple and reproducible assay with a great price.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.