ZAG is a 41 kDa soluble glycoprotein, which is a homologue of the class 1 major histocompatibility complex (MHC) heavy chain. ZAG was found to stimulate lipolysis through stimulation of adenylyl cyclase in a GTP-dependent process. ZAG produced weight loss when administered to obese mice without a reduction in food and water intake. Loss of body weight was entirely due to loss of adipose tissue with no effect on lean body mass. There was a three-fold increase in oxygen consumption by interscapular brown adipose tissue (BAT), suggesting an increase in thermogenesis. Recent studies have reported that ZAG is not only capable of stimulating lipolysis through activation of HSL, but also increases the utilization of the released NEFA through an increased level of UCPs in BAT and skeletal muscle.

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Standard, Biotinylated Zinc alpha 2-glycoprotein (ZAG) peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.