Human MCP-2 ELISA

$398.00
ELH-MCP2-1

RayBio® Human MCP-2 (CCL8) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
1.5 pg/ml

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Detection Range
1.5 pg/ml - 200 pg/ml
Recommended Dilution (serum & plasma)
2 - 5 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP-1, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Species Detected
Human
Protein Name & Synonyms
C-C motif chemokine 8 (HC14) (Monocyte chemoattractant protein 2) (Monocyte chemotactic protein 2) (MCP-2) (Small-inducible cytokine A8)
Accession Number
Gene Names
CCL8, MCP2, SCYA10, SCYA8
Gene ID
6355

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • CCL8

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Human
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Petrosino S., Puigdemont A., Della Valle M., et al. Adelmidrol increases the endogenous concentrations of palmitoylethanolamide in canine keratinocytes and down-regulates an inflammatory reaction in an in vitro model of contact allergic dermatitis. Vet J. 2016 Jan;207:85-91. doi: 10.1016/j.tvjl.2015.10.060.
    Species: Human
    Sample Type: Conditioned Media

  2. Ahrens B., Schulz G., Bellach J., et al. Chemokine levels in serum of children with atopic dermatitis with regard to severity and sensitization status. Pediatr Allergy Immunol. 2015 Nov;26(7):634-40. doi: 10.1111/pai.12431.
    Species: Human
    Sample Type: Serum

  3. Poole E., et al. Latency-associated viral IL-10 encoded by human cytomegalovirus modulates cellular IL-10 and CCL8 secretion during latent infection through changes in the cellular miRNA, has miR-92a. Journal of Virology, Sept 2014 epub ahead of print. doi:10.1128/JVI.02424-14
    Species: Human
    Sample Type: Conditioned Media

  4. Hamilton SA, Tower CL, Jones RL. Identification of chemokines associated with the recruitment of decidual leukocytes in human labour: potential novel targets for preterm labour. PLoS ONE. 2013;8(2):e56946.
    Species: Human
    Sample Type: Tissue Lysate

  5. Liu H, Liu Z, Chen J, et al. Induction of CCL8/MCP-2 by mycobacteria through the activation of TLR2/PI3K/Akt signaling pathway. PLoS ONE. 2013;8(2):e56815.
    Species: Human
    Sample Type: Pleural Fluid

  6. Chen YL., Huang KF., Kuo WC., et al. Inhibition of glutaminyl cyclase attenuates cell migration modulated by monocyte chemoattractant proteins. Biochem. J. (2012) 442, 403-412. doi:10.1042/BJ20110535
    Species: Human
    Sample Type: Conditioned Media

  7. Wang D, Prakash J, Nguyen P, et al. Bone Morphogenetic Protein Signaling in Vascular Disease: ANTI-INFLAMMATORY ACTION THROUGH MYOCARDIN-RELATED TRANSCRIPTION FACTOR A. The Journal of Biological Chemistry 2012;287(33):28067-28077. doi:10.1074/jbc.M112.379487.
    Species: Human
    Sample Type: Conditioned Media

  8. Borden, Ernest C., et al. "Gene regulatory and clinical effects of interferon β in patients with metastatic melanoma: a phase II trial." Journal of Interferon & Cytokine Research 31.5 (2011): 433-440.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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