NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA.p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.

Greater than 95% as determined by SDS-PAGE.

Sterile Filtered White lyophilized (freeze-dried) powder.

The protein was lyophilized from 0.5xPBS, pH 7.5.

IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.

Description

Interleukin-12 Mouse Recombinant produced in Sf9 insect cells is a glycosylated disulfide linked heterodimeric polypeptide containing 506 amino acids and having a molecular weight of 75 kDa comprised of disulfide-bonded 35 kDa (p35) and 40 kDa (p40) subunits.The IL-12 is purified by proprietary chromatographic techniques.

Stability & Storage

Storage/Stability

Lyophilized mouse IL-12 although stable at room temperature for 3 weeks, should be stored below -18°C. Upon reconstitution IL12 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.

Greater than 95% as determined by SDS-PAGE.

Sterile Filtered White lyophilized (freeze-dried) powder.

The protein was lyophilized from 0.5xPBS, pH 7.5.

Description

Stability & Storage

Storage/Stability

Size	10 µg, 100 µg
Estimated Lead Time	2-3 weeks
Species	Mouse
Protein Name / Synonyms	NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Expressed Region	p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA.p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.
Expression System	HEK293 cells
Purity	Greater than 95% as determined by SDS-PAGE.
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The protein was lyophilized from 0.5xPBS, pH 7.5.
Activity	IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.
Research Area	Inflammation
Shipping Type	Ambient temperature
Storage	-20°C

Size	10 µg, 100 µg
Estimated Lead Time	2-3 weeks
Species	Mouse
Protein Name / Synonyms	NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Edodekin-alpha, IL-12.
Expressed Region	p35 Subunit: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA.p40 Subunit: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS.
Expression System	HEK293 cells
Purity	Greater than 95% as determined by SDS-PAGE.
Format	Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation	The protein was lyophilized from 0.5xPBS, pH 7.5.
Activity	IL-12 Mouse has full biological activity when compared to standards. The ED50 is determined by the dose-dependent induced expression of CD25 on CD8+ mouse T cells is 10-20 ng/ml corresponding to a specific activity of 50,000-100,000units/mg.
Research Area	Inflammation
Shipping Type	Ambient temperature
Storage	-20°C

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Interleukin-12, Mouse Recombinant

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Documents

Reviews

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Interleukin-12, Mouse Recombinant

Interleukin-12, Mouse Recombinant