L-Series: Label-Based Antibody Arrays

Semi-quantitative, Label-based, High Density Antibody Arrays

Utilizing direct antigen-labeling technology, RayBiotech has enabled the largest commercially available antibody array to date. With the RayBio^® Biotin Label-based Antibody Array (“L-Series”) researchers can obtain a broad, panoramic view of protein expression. Up to 1000 target proteins can be simultaneously detected, including cytokines, chemokines, adipokines, growth factors, proteases, soluble receptors, adhesion molecules, and other proteins, making this array ideally suited for biomarker discovery studies and exploratory screens.

Through a simple labeling process, the sample proteins are directly conjugated to biotin, eliminating the need for a second antibody to develop the array signals. In this format, unintended antibody interactions are impossible, thus eliminating limitations on the size of the array panel.

For a comparison & overview of all our antibody array systems, click here.

Compare L-Series Protein Lists Browse L-Series Arrays

Features

Highest density screening array available
Available in both membrane and glass slide format
Ultra high detection sensitivity
Reactive with multiple species (high cross-species reactivity)
Accurate and reproducible
Affordable, quick and simple to use
Validated for serum, plasma, and cell culture media

Research Applications

High throughput expression profiling
Discovering potential molecular targets for drug development
Uncovering molecular mechanisms of drug action
Identifying crucial factors involved in disease processes
Discovering biomarkers for disease management
Discovering expression patterns for molecular classification of diseases

How It Works

Illustration of Label-Based Detection

Our RayBio^® Label-Based Arrays differ from our sandwich ELISA-based arrays primarily in the means of detection. Instead of using a second biotinylated antigen-specific antibody, sample proteins themselves are labeled with biotin prior to incubation with the capture antibodies. Proteins binding to the array can be detected using streptavidin-HRP for chemiluminescence detection or streptavidin-conjugated HiLyte Fluor 532™ for fluorescence detection.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

Contents of Kit

RayBio^® L-Series Antibody Array Membranes or Glass Slides
Spin Columns / Dialysis Tube
Labeling Reagent
Stop Solution
Blocking Buffer
Streptavidin-Conjugated HRP or Streptavidin-Conjugated HiLyte Fluor 532
Wash Buffer 1
Wash Buffer 2
Plastic Sheets
Floating Dialysis Rack
Plastic Incubation Tray
Detection Buffer C
Detection Buffer D

*Accessories include: 2-well or 4-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

Assay Diagram

The cell culture supernates or serum is dialyzed before biotin-labeling step. Through a simple process, the primary amine of the proteins in the sample is biotinylated, followed by dialysis to remove free biotin. From here, the newly biotinylated sample is added onto the array membrane or glass slide and incubated at room temperature. After incubation with HRP-streptavidin or Fluorescent Dye-Strepavidin, the signals can be visualized either by chemiluminescence or fluorescence. The experimental procedure is simple and can be performed in any laboratory.

Representative Data

A) Human L-507 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio^® Biotin-label-based Human Antibody Array 507 probed with two cell culture supernates. The image were captured using a Axon GenePix laser scanner.