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L-Series: Label-Based Antibody Arrays

Semi-quantitative, Label-based, High Density Antibody Arrays

Introducing the Human L2000 Array Simultaneously detect 2000 proteins using our largest array yet

Combining direct antigen-labeling technology with our vast library of array-validated antibodies, RayBiotech has created our largest antibody array to date: the Human L2000 Array. With the L-Series high density array platform, researchers can now detect up to 2000 proteins simultaneously, obtaining a broad, panoramic view of protein expression. Our newly expanded panel includes a wide variety of metabolic enzymes, structural proteins, epigenetic markers, neuroregulatory factors, in addition to our popular list of cytokines, growth factors, receptors, adipokines, proteases, and signaling proteins. Available on both glass slide and membrane formats, this array is ideally suited for biomarker discovery studies and exploratory screens.

For a comparison & overview of all our antibody array systems, click here.

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Features

  • High detection sensitivity
  • Easy to use; obtain results in 2 days
  • No need for fractionation, depletion or extraction of samples
  • Consumes only 20-30 ul of serum/plasma
  • No dedicated equipment needed (glass slide or membrane)
  • Accurate and reproducible
  • As little as 59¢ per target

Research Applications

  • High throughput expression profiling
  • Discovering potential molecular targets for drug development
  • Uncovering molecular mechanisms of drug action
  • Identifying crucial factors involved in disease processes
  • Discovering biomarkers for disease management
  • Discovering expression patterns for molecular classification of diseases

How It Works

Through a simple labeling process, the sample proteins are directly conjugated to biotin, eliminating the need for a second antibody to develop the array signals. In this format, unintended antibody interactions are impossible, thus eliminating limitations on the size of the array panel.

Illustration of Label-Based Detection

Our RayBio® Label-Based Arrays differ from our sandwich ELISA-based arrays primarily in the means of detection. Instead of using a second biotinylated antigen-specific antibody, sample proteins themselves are labeled with biotin prior to incubation with the capture antibodies. Proteins binding to the array can be detected using streptavidin-HRP for chemiluminescence detection or streptavidin-conjugated HiLyte Fluor 532™ for fluorescence detection.

For a list of laser scanner specifications, click here.

If you do not have access to a scanner, RayBiotech provides slide scanning and data analysis services

Contents of Kit

  • RayBio® L-Series Antibody Array Membranes or Glass Slides
  • Spin Columns / Dialysis Tube
  • Labeling Reagent
  • Stop Solution
  • Blocking Buffer
  • Streptavidin-Conjugated HRP or Streptavidin-Conjugated HiLyte Fluor 532
  • Wash Buffer 1
  • Wash Buffer 2
  • Plastic Sheets
  • Floating Dialysis Rack
  • Plastic Incubation Tray
  • Detection Buffer C
  • Detection Buffer D

*Accessories include: 2-well or 4-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film

Assay Diagram

The cell culture supernates or serum is dialyzed before biotin-labeling step. Through a simple process, the primary amine of the proteins in the sample is biotinylated, followed by dialysis to remove free biotin. From here, the newly biotinylated sample is added onto the array membrane or glass slide and incubated at room temperature. After incubation with HRP-streptavidin or Fluorescent Dye-Strepavidin, the signals can be visualized either by chemiluminescence or fluorescence. The experimental procedure is simple and can be performed in any laboratory.

Representative Data

A) Human L-507 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio® Biotin-label-based Human Antibody Array 507 probed with two cell culture supernates. The image were captured using a Axon GenePix laser scanner.

B) Mouse L-308 Array (Glass Slide Format | Membrane Format)

The figure shows the RayBio® Biotin-label-based Mouse Array 308 probed with Mouse sample. The image were captured using a Axon GenePix laser scanner.

References

  1. Ryu JC, Davidson BP, xie A, et al. Molecular imaging of the paracrine proangiogenic effects of progenitor cell therapy in limb ischemia. Circulation. 2013 Feb 12;127(6):710-719.
    Species: Human
    Sample Type:Conditioned Media
  2. Toh H, Wang W, Chia W, Kvistborg P, Sun Li,et al. Clinical Benefit of Allogeneic Melanoma Cell Lysate-Pulsed Autologous Dendritic Cell Vaccine in MAGE-Positive Colorectal Cancer Patients.Clin Cancer Res. 2009;15(24):7726-7736
    Species: Human
    Sample Type: Plasma
  3. Jiang H., et al. A pilot study of angiogenin in heart failure with preserved ejection fraction: a novel potential biomarker for diagnosis and prognosis? Journal of cellular and molecular medicine Aug 15, 2014. Epub ahead of print. DOI: 10.1111/jcmm.12344
    Species: Human
    Sample Type: Serum
  4. Yousef H., Conboy M., Mamiya H., Zeiderman M., Schlesinger C., Schagger D., Conboy I. Mechanisms of action of hESC-secreted proteins that enhance human and mouse myogensis. Aging vol. 6 no 5.
    Species: Human
    Sample Type: Conditioned Media
  5. Kim N, Dabrowska A., Jenner R.G., Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species, J Virol. Jul 2007; 81(14): 7584–7597
    Species: Non-Human Primate
    Sample Type: Conditioned Media
  6. Chesnokova, Vera, Cuiqi Zhou, Anat Ben-Shlomo, Svetlana Zonis, Yuji Tani, Song-Guang Ren, and Shlomo Melmed. "Growth Hormone Is a Cellular Senescence Target in Pituitary and Nonpituitary Cells." PNAS. NCBI, 23 July 2013. Web.
    Species: Human
    Sample Type: Conditioned Media
  7. Zhong J., et al. Temporal Profiling of the Secretome during Adipogenesis in Humans. J. Proteome Res., 2010, 9 (10), pp 5228-5238
    Species: Human
    Sample Type: Conditioned Media
  8. Palumbo S, Li W-J. Osteoprotegerin Enhances Osteogenesis of Human Mesenchymal Stem Cells. Tissue Eng Part A. Volume 19, Numbers 19 and 20, 2013, DOI: 10.1089/ten.tea.2012.0550
    Species: Human
    Sample Type: Conditioned Media
  9. Tang YL, Zhu WW, Cheng M, Chen LJ, Zhang J, et al. Hypoxic Preconditioning Enhances the Benefit of Cardiac Progenitor Cell Therapy for Treatment of Myocardial Infarction by Inducing CXCR4 Expression. Circ Res. 2009;104:1209-1216. DOI: 10.1161/CIRCRESAHA.109.197723
    Species: Mouse
    Sample Type: Conditioned Media
  10. Vause, C. V. and Durham, P. L. (2012), Identification of Cytokines and Signaling Proteins Differentially Regulated by Sumatriptan/Naproxen. Headache: The Journal of Head and Face Pain, 52: 80-89. doi: 10.1111/j.1526-4610.2011.02048.x
    Species: Rat
    Sample Type: Tissue Lysate

More references are listed per product on the individual product pages.

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