The improved guanidine isothiocyanate/phenol one-step method (TRIzol LS (liquid sample) method) lyses cells and inactivates RNases. Total RNA is then selectively adsorbed to the silica membrane in the spin column under high salt conditions. A series of rapid wash-centrifugation steps using deproteinization and wash solutions then removes cellular debris, proteins, and other impurities. Finally, RNase-free water with low salt concentration elutes the purified RNA from the silica membrane.

Store TRIpure LS Reagent at 4°C and rest of kit components at room temperature. Stable for 6 months if stored properly.