Lung Cancer IgG Autoantibody Array G1

RayBio® Lung Cancer IgG Autoantibody Array G1 contains 30 human proteins for simultaneous detection of human IgG autoantibodies associated with lung cancer.

$300.73
PAH-LCAG-G1-8
+

Assay Format

Number of Targets Detected:
30
Species Detected:
  • Human
Compatible Sample Types:
  • Hydrothorax
  • Plasma
  • Serum
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
8 or 16 Sample Kit

Product Feature

  • Small sample volume required (2 µl)
  • Detects antibody specificity
  • Detects autoantibody expression
  • Affordable, quick and simple to use

Target Name

Scroll over each spotted protein for more information

14-3-3 theta
  • Gene Symbol: YWHAQ
  • Gene ID: 10971
  • Accession #: P27348
Annexin A1
Annexin A2
  • Gene Symbol: ANXA2 ANX2 ANX2L4 CAL1H LPC2D
  • Gene ID: 302
  • Accession #: P07355
CAGE
  • Gene Symbol: DDX53 CAGE
  • Gene ID: 168400
  • Accession #: Q86TM3
Cathepsin D
CDK2
  • Gene Symbol: CDK2 CDKN2
  • Gene ID: 1017
  • Accession #: P24941
c-Myc
(MYC)
Cyclin A
  • Gene Symbol: CCNA2 CCN1 CCNA
  • Gene ID: 890
  • Accession #: P20248
Cyclin B1
  • Gene Symbol: CCNB1 CCNB
  • Gene ID: 891
  • Accession #: P14635
Cyclin D1
  • Gene Symbol: CCND1
  • Accession #: Q6FI00
DKK-1
GBU4-5
  • Gene Symbol: TDRD12
  • Gene ID: 25044
  • Accession #: Q587J7
HSP70-9B/HSPA9/GRP-75
  • Gene Symbol: HSPA9 GRP75 HSPA9B mt-HSP70
  • Gene ID: 3313
  • Accession #: P38646
Hu-D
  • Gene Symbol: ELAVL4 HUD PNEM
  • Gene ID: 1996
  • Accession #: P26378
IMDH2
  • Gene Symbol: IMPDH2 IMPD2
  • Gene ID: 3615
  • Accession #: P12268
IMP1
  • Gene Symbol: IGF2BP1 CRDBP VICKZ1 ZBP1
  • Gene ID: 10642
  • Accession #: Q9NZI8
IMP2/IGF2BP2/p62
  • Gene Symbol: IGF2BP2 IMP2 VICKZ2
  • Gene ID: 10644
  • Accession #: Q9Y6M1
IMP3
  • Gene Symbol: IGF2BP3 IMP3 KOC1 VICKZ3
  • Gene ID: 10643
  • Accession #: O00425
LAMR1
  • Gene Symbol: RPSA LAMBR LAMR1
  • Gene ID: 3921
  • Accession #: P08865
MAGEA4
  • Gene Symbol: MAGEA4 MAGE4
  • Gene ID: 4103
  • Accession #: P43358
MALAT1
  • Gene Symbol: MALAT1
  • Gene ID: 29665
  • Accession #: Q9UHZ2
NY-ESO-1
  • Gene Symbol: CTAG1A CTAG CTAG1 ESO1 LAGE2 LAGE2A; CTAG1B LAGE2B
  • Gene ID: 1485; 246100
  • Accession #: P78358
p53
Paxillin
  • Gene Symbol: PXN
  • Gene ID: 9718
  • Accession #: P49023-2
PGAM1
  • Gene Symbol: PGAM1 PGAMA CDABP0006
  • Gene ID: 5223
  • Accession #: P18669
PGP9.5
  • Gene Symbol: UCHL1
  • Gene ID: 7345
  • Accession #: P09936
Recoverin
  • Gene Symbol: RCVRN RCV1
  • Gene ID: 5957
  • Accession #: P35243
SOX2
Survivin
(BIRC5)
  • Gene Symbol: BIRC5 API4 IAP4
  • Gene ID: 332
  • Accession #: O15392
Ubiquilin-1
  • Gene Symbol: UBQLN1 DA41 PLIC1
  • Gene ID: 29979
  • Accession #: Q9UMX0

Application Notes

Suggested Application
Lung cancer IgG autoantibody profiling
Kit Components
  • RayBio® Human Lung Cancer IgG Autoantibody G1 Array Slide
  • RayBio® G series antibody array accessory (including slide incubation chamber assembly, Gasket, Protective cover, Snap-on sides and adhesive film)
  • Blocking Buffer (8 ml/bottle)
  • Wash Buffer 1 (30 ml/bottle)
  • Wash Buffer 2 (30 ml/bottle)
  • 1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)
  • 1,500X HiLyte 555 Streptavidin (1 µl/vial)
Other Materials Required
  • Small plastic boxes or containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • Distilled water
  • Aluminum foil
  • Laser scanner for fluorescence detection
Protocol Outline
  1. See Manual for relevant protocols.

Typical Data

The following image is a typical result of the Human Lung Cancer Autoantibody Protein Array. The arrays were incubated with 1:200 diluted serum samples. The results shown in Fig. A indicate no background from the biotin-labeled anti-human IgG antibody or from the fluorescent dye-labeled streptavidin. In Fig. B, a base background level is established from normal serum. This background can be subtracted during data analysis without affecting the results. Lastly, Figures C and D are the results of two individuals diagnosed with lung cancer. Several autoantibodies show clear binding.

Fig 1. Human serum test of RayBio® Lung Cancer Autoantibody Detection Array. The protein arrays were respectively incubated with either no serum (A) or with diluted serum samples derived from either normal human (B), lung cancer patient A (C), or lung cancer patient B (D). Biotin labeled BSA positive controls are outlined in red. Human IgG positive controls are outlined in white.

Storage/Stability

Upon receipt, all components of the RayBio® Human Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass slide and Blocking Buffer should be kept at –20 °C and all other component should be stored at 4 °C Use within three months after reagents have been thawed.

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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $416.88
Price for all $335.57

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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