Monkeypox Virus (MPXV) A29L ELISA Kit

The RayBiotech Monkeypox Virus (MPXV) A29L ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of MPXV protein A29L in serum, plasma, and cell culture supernatants.

Lead time: Typically ships within 1-2 business days. No Friday shipments.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Monkeypox virus (MPXV)
Sensitivity	3.6 pg/ml
Detection Range	3.6 pg/ml - 20 ng/ml
Specificity	This ELISA antibody pair detects MPXV A29L.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Recommended Dilution (Serum/Plasma)	3 fold
Accession Number	Q90188
Gene Symbols	A29L, MPXV-M5312_HM12_Rivers-138
Protein Name / Synonyms	14 kDa protein (14K membrane protein) (14kDa fusion protein) (MPXVgp139) (A29L)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Infectious Disease, Mpox
Shipping Type	Blue ice
Storage	-20°C

Introduction

This assay employs an antibody specific for MPXV A29L coated on a 96-well plate. Standards and samples are pipetted into the wells and MPXV A29L present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-MPXV A29L antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MPXV A29L bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Monkeypox Virus (MPXV) A29L ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified A29, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (ELV-MPXVA29.pdf)

SDS (ELV-MPXVA29.pdf)

Introduction

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Customize This Monkeypox Virus (MPXV) A29L ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified A29, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Featured Products

Monkeypox Virus (MPXV) A29L ELISA Kit

Product Description

Specifications

Introduction

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Storage/Stability

Customize This Monkeypox Virus (MPXV) A29L ELISA

Have this product in hand?

Featured Products

Monkeypox Virus (MPXV) A29L ELISA KitMonkeypox Virus (MPXV) A29L ELISA Kit

Product Description

Specifications

Introduction

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Storage/Stability

Customize This Monkeypox Virus (MPXV) A29L ELISA

Have this product in hand?

Monkeypox Virus (MPXV) A29L ELISA Kit

Monkeypox Virus (MPXV) A29L ELISA KitMonkeypox Virus (MPXV) A29L ELISA Kit

Featured Products

Monkeypox Virus (MPXV) A29L ELISA Kit

Product Description

Specifications

Introduction

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Storage/Stability

Customize This Monkeypox Virus (MPXV) A29L ELISA

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Monkeypox Virus (MPXV) A29L ELISA KitMonkeypox Virus (MPXV) A29L ELISA Kit

Product Description

Specifications

Introduction

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Storage/Stability

Customize This Monkeypox Virus (MPXV) A29L ELISA

Documents

Have this product in hand?

Reviews

Ask A Question

Monkeypox Virus (MPXV) A29L ELISA Kit

Monkeypox Virus (MPXV) A29L ELISA KitMonkeypox Virus (MPXV) A29L ELISA Kit