Monkeypox Virus Proteins

Recombinant Monkeypox Antigens

Introduction

Monkeypox Virus (MPXV) is an enveloped double-stranded DNA virus that belongs to the genus Orthopoxvirus in the family Poxviridae. While monkeypox was first discovered in the colonies of monkey kept for research in 1958, first human case of monkeypox was registered in 1970. Monkeypox is thought to be endemic to West Africa and Central Africa. In 2003, there was a monkeypox outbreak outside of Africa and it occurred in the United States of America. Recently, monkeypox cases have been reported in multiple non-endemic countries raising awareness.

The 196,858-bp monkeypox virus genome consists of ~200 open reading frames with ≥60 amino acid residues. RayBiotech now offers 15 bacterial- or mammalian-derived monkeypox proteins. Recombinant proteins are key components in current infectious disease research. They are widely used for antibody and vaccine development and as key components in immunodiagnostic assays.

Diagram of the Monkeypox virus structure

Products

RayBiotech offers a comprehensive catalog of recombinant Monkeypox virus proteins derived from mammalian cells and bacteria.

Full Protein Name Short Protein Name Catalog # Expression Host Expression Region Tag
IMV Surface Membrane 14-kDa Fusion Protein A29L 230-01204 E.coli Met1-Glu110 N-terminal His-tag
IMV Surface Membrane 14-kDa Fusion Protein A29L 230-30237 HEK293 Met1-Glu110 C-terminal His-tag
Envelope protein A28 homolog A30L 230-01201 E.coli Gln22-Leu146 N-terminal His-tag
Envelope protein A28 homolog A30L 230-30235 HEK293 Gln22-Leu146 C-terminal His-tag
EEV envelope glycoprotein A35R 230-01203 E.coli Gln59-Thr181 N-terminal His-tag
EEV Envelope Glycoprotein A35R 230-30238 HEK293 Gln59-Thr181 C-terminal His-tag
IFN-alpha/beta receptor-like Glycoprotein B16R B16R 230-01193 E. coli His21-Glu352 N-terminal His-tag
IFN-alpha/beta receptor-like Glycoprotein B16R B16R 230-30236 HEK293 His21-Glu352 C-terminal His-tag
Palmytilated EEV Membrane Glycoprotein C19L 230-01196 E. coli Met1-Phe307 N-terminal His-tag
Secreted IL-18 Binding Protein D6L D6L 230-01194 E. coli Met1-Lys126 N-terminal His-tag
Secreted IL-18 Binding Protein D6L D6L 230-30234 HEK293 Met1-Lys126 C-terminal His-tag
Cell Surface-binding Protein E8L E8L 230-01195 E. coli Met1-Thr275 N-terminal His-tag
Cell Surface-binding Protein E8L E8L 230-30232 HEK293 Met1-Thr275 C-terminal His-tag
Envelope Protein H3L H3L 230-01192 E. coli Met1-Pro278 N-terminal His-tag
Envelope Protein H3L H3L 230-30233 HEK293 Met1-Pro278 C-terminal His-tag
Telomere-binding Protein I1 I1L 230-01198 E. coli Met1-Glu312 N-terminal His-tag
L1R protein, required for DNA packaging L1R 230-01202 E. coli Met1-Gln152 N-terminal His-tag

Typical Data

SDS-PAGE Image of Recombinant Monkeypox Virus Cell Surface-binding Protein, E8L

SDS-PAGE image of recombinant monkeypox protein E8L

Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.

  1. Lane 1: Protein standard ladder (kDa).
  2. Lane 2: Untreated protein.
  3. Lane 3: Treated protein with deglycosylation enzymes under native conditions.
  4. Lane 4: Treated protein with deglycosylation enzymes under denature conditions.

References

  1. Shchelkunov, SN, et al. Analysis of the Monkeypox Virus Genome. Virology, (2002) 297, 172–194.
  2. Haifeng Song, et al. Characterizing monkeypox virus specific CD8+T cell epitopes in rhesus macaques. Virology, (2013) 447(0): 181–186.
  3. Townsend BT, et al, Humoral Immunity to Smallpox Vaccines and Monkeypox Virus Challenge: Proteomic Assessment and Clinical Correlations. Journal of Virology, 87(2): 900–911
  4. Jessica R. Weaver and Stuart N. Isaacs. Monkeypox virus and insights into its immunomodulatory proteins. Immunol Rev. (2008) 225: 96–113

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