Mpox Virus Proteins
Mpox Virus (MPXV) is an enveloped double-stranded DNA virus that belongs to the genus Orthopoxvirus in the family Poxviridae. While mpox was first discovered in the colonies of monkey kept for research in 1958, first human case of mpox was registered in 1970. Mpox is thought to be endemic to West Africa and Central Africa. In 2003, there was a mpox outbreak outside of Africa and it occurred in the United States of America. Recently, mpox cases have been reported in multiple non-endemic countries raising awareness.
The 196,858-bp mpox virus genome consists of ~200 open reading frames with ≥60 amino acid residues. RayBiotech now offers 15 bacterial- or mammalian-derived mpox proteins. Recombinant proteins are key components in current infectious disease research. They are widely used for antibody and vaccine development and as key components in immunodiagnostic assays.
SDS-PAGE of Recombinant mpox Virus Cell Surface-binding Protein, E8L
Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
- Lane 1: protein standard ladder (kDa).
- Lane 2: untreated protein.
- Lane 3: treated protein with deglycosylation enzymes under native conditions.
- Lane 4: treated protein with deglycosylation enzymes under denature conditions.
