Recombinant Monkeypox virus (MPXV) cell surface-binding protein E8L with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 100 µg, 250 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Monkeypox virus (MPXV) |
| Accession Number | Q8V4Y0 |
| Gene Symbols | E8L |
| Protein Name / Synonyms | Cell surface-binding protein, carbonic anhydrase homolog, E8L |
| Expressed Region | Met1-Thr275 |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | >95% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼32 kDa. Due to the abundant glycosylation, it migrates as approximately ∼40 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS (pH 7.4) with 1% mannitol and 5% trehalose |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1 mg/mL with sterile deionized water. |
| Endotoxin Level | <0.5 EU per ug of the protein as determined by the LAL method |
| Research Area | Infectious Disease, Mpox |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
Introduction
Monkeypox Virus Cell Surface-Binding Protein E8L is an envelope protein encoded by the monkeypox virus, a member of the Orthopoxvirus genus. The E8L protein is involved in the virus's ability to bind to host cell surfaces, facilitating viral entry and infection. It is considered a potential target for diagnostic assays and therapeutic interventions due to its role in the initial stages of monkeypox virus infection.
SDS-PAGE Image
Lane 1: protein standard ladder (kDa).
Lane 2: untreated protein.
Lane 3: treated protein with deglycosylation enzymes under native conditions.
Lane 4: treated protein with deglycosylation enzymes under denature conditions.
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
