Mouse Anti-Human BMI1 Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Accession Number | P35226 |
| Gene Id | 648 |
| Gene Symbols | BMI1 |
| Protein Name / Synonyms | BMI1 |
| Clonality | Monoclonal |
| Preparation | This BMI1 monoclonal antibody is generated from mouse immunized with BMI1 recombinant protein. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility. In the PRC1 complex, it is required to stimulate the E3 ubiquitin-protein ligase activity of RNF2/RING2.
Specificity
Other Names
BMI1; PCGF4; RNF51; Polycomb complex protein BMI-1; Polycomb group RING finger protein 4; RING finger protein 51
NCBI Accession #
NP_001190991.1;NP_005171.4
Other Accession #
NP_005171.4
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
36949
Recommended Dilutions
WB: 1:100-250; IF: 1:10-50
 | BMI1 Antibody (Cat# 102-10158) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the BMI1 antibody detected the BMI1 protein (arrow). |
 | Confocal immunofluorescent analysis of BMI1 Antibody (Cat# 102-10158) with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue). |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.