Protein Name & Synonyms:
Flow Cytometry, Immunoprecipitation, Western Blotting
IgG concentration 1.0 mg/ml
Human, Rhesus monkey and guinea pig
This product recognizes the human Toll like receptor 4 (TLR4) cell surface antigen.
TLR4, also known as CD284, has been demonstrated to act as a receptor for LPS on human monocytes and macrophages. TLR4 signalling of LPS stimulation requires the presence of the MD-2 molecule. TLR4 is weakly expressed by resting cells, but is upregulated following stimulation with LPS.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Ba/F3 cell line expressing TLR4 (CD284)
Spleen cells from immunised Balb/c mice were fused with cells of the mouse SP2/0 myeloma cell line.