Mouse Anti-Human EIF4E Antibody, 400 ul
$334.00
In stock
Code:
102-10076
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+

Product Description

Specifications

Size 400 µl
Protein Name / Synonyms EIF4E
Accession Number P06730
Gene Symbols EIF4E
Gene Id 1977
Preparation This EIF4E monoclonal antibody is generated from mouse immunized with EIF4E recombinant protein.
Clonality Monoclonal
Species Human

Introduction

EIF4E recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation of protein synthesis and facilitates ribosome binding by inducing the unwinding of the mRNAs secondary structures.

Specificity

Other Names
EIF4E; EIF4EL1; EIF4F; Eukaryotic translation initiation factor 4E; eIF-4F 25 kDa subunit; mRNA cap-binding protein
NCBI Accession #
NP_001124150.1;NP_001124151.1;NP_001959.1
Other Accession #
NP_001959.1

Format

Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
25097
Recommended Dilutions
IF: 1:10-50; WB: 1:100-500
Fluorescent image of Hela cell stained with EIF4E Antibody(Cat# 102-10076/SG101020AF).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with EIF4E primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). EIF4E immunoreactivity is localized to Cytoplasm significantly.
EIF4E Antibody (Cat# 102-10076) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EIF4E antibody detected the EIF4E protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
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