Protein Name & Synonyms:
GAPDH
Applications:
Immunohistology - Frozen, ELISA, Immunofluorescence, Immunoprecipitation, Western Blotting
Concentration (lot specific):
1.0mg/ml
Species Reactivity:
Human, Bovine, Cat, Dog, Fish, Goat, Mouse, Pig, Rabbit, Rat
This product recognizes glyceraldehyde-3-phosphate dehydrogenase (GAPDH), a 36kD multifunctional protein whose main function is to catalyse the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate, in conjunction with inorganic phosphate and nicotinamide adenine dinucleotide (NAD). This reaction is an important energy yielding step in carbohydrate metabolism.
GAPDH has also been shown to translocate to the nucleus under a variety of stressors, most of which are associated with oxidative stress, whereby it mediates cell death. A further report has shown that GAPDH binds to several proteins that are responsible for neurodegenerative diseases, such as amyloid precursor protein and Huntingtin.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
Purified IgG - liquid
Formulation:
Phosphate buffered saline
Immunogen:
Human cardiac muscle GADPH
Preservative:
0.09% Sodium Azide (NaN3)
This product
Spleen cells from immunised Balb/c mice were fused with cells of the Sp2/0 myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.