Protein Name & Synonyms:
Immunohistology - Frozen, ELISA (dilution: 1/500 , 1/1000), Flow Cytometry, Immunohistology - Paraffin, Western Blotting
Concentration (lot specific):
IgG concentration 1.0 mg/ml
This product recognizes human myeloperoxidase (MPO). MPO is an important component of azurophilic granules in neutrophils, being involved in microbicidal processes. The protein is a multimer of 2 heavy chains (55kD) and two light chains (15kD), the heavy chains being linked by a disulphide bond. This product recognizes native MPO in Western blots, and the heavy chain following boiling of the sample. The antibody also recognizes recombinant MPO in Western blots and weakly in ELISA.
The antibody may be of value in the study of myeloid cells and myeloid leukaemias by flow cytometry following cell permeablisation.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Spleen cells from immunised mice were fused with cells of the mouse X63 AG8-653 myeloma cell line.
12 months from the date of shipment when stored properly.