Protein Name & Synonyms:
ELISA, Immunoprecipitation, Western Blotting
This product recognizes human pregnancy-associated plasma protein A, or PAPP-A, a metalloprotease specific for insulin-like growth factor binding protein-4 and -5 (IGFBP4 and IGFBP5). PAPP-A was initially identified as a high-molecular mass component in human pregnancy serum. In pregnancy serum PAPP-A exists in a disulfide bound heterotetrameric complex consisting of two 200kDa PAPP-A subunits and two 50-90kDa subunits of the proform of eosinophil major basic protein (proMBP). The concentration of PAPP-A in serum increases with gestational age, however, in Down's syndrome pregnancies PAPP-A concentration is markedly reduced in the first trimester. As such, PAPP-A is widely used as an early marker for Down's syndrome.
PAPP-A has also been shown to exist in a homodimeric, 400kDa form in unstable atherosclerotic plaques. Increased circulating concentrations of this dimeric PAPP-A are associated with acute coronary syndromes.
This product recognizes both the heterotetrameric complex (PAPP-A associated with proMBP) and the homodimeric complex of PAPP-A.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Purified IgG - liquid
Phosphate buffered saline
Purified human PAPP-A/proMBP complex
Spleen cells from immunised BALB/c mice were fused with cells of the Sp2/0 myeloma cell line.