This TRAF2 monoclonal antibody is generated from mouse immunized with TRAF2 recombinant protein.
Clonality
Monoclonal
Species
Human
Introduction
The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from members of the TNF receptor superfamily. This protein directly interacts with TNF receptors, and forms a heterodimeric complex with TRAF1. This protein is required for TNF-alpha-mediated activation of MAPK8/JNK and NF-kappaB. The protein complex formed by this protein and TRAF1 interacts with the inhibitor-of-apoptosis proteins (IAPs), and functions as a mediator of the anti-apoptotic signals from TNF receptors. The interaction of this protein with TRADD, a TNF receptor associated apoptotic signal transducer, ensures the recruitment of IAPs for the direct inhibition of caspase activation. BIRC2/c-IAP1, an apoptosis inhibitor possessing ubiquitin ligase activity, can unbiquitinate and induce the degradation of this protein, and thus potentiate TNF-induced apoptosis. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of only one transcript has been determined.
Specificity
Other Names
TRAF2; TRAP3; TNF receptor-associated factor 2; E3 ubiquitin-protein ligase TRAF2; Tumor necrosis factor type 2 receptor-associated protein 3
NCBI Accession #
NP_066961.2
Other Accession #
NP_066961.2
Format
Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
55859
Recommended Dilutions
IF: 1:10-50; WB: 1:100-1000
Confocal immunofluorescent analysis of TRAF2 Antibody (Cat# 102-10124) with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-mouse lgG (green). DAPI was used to stain the cell nuclear (blue).
TRAF2/MB10188 antibody (Cat# 102-10124) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the TRAF2/MB10188 antibody detected the TRAF2/MB10188 protein (arrow).
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
