Protein Name & Synonyms:
CD90
Applications:
Cytotoxic Assays, Flow Cytometry
Concentration (lot specific):
IgM concentration 1.0 mg/ml
Preparation:
IgM fraction prepared by ammonium sulfate precipitation
Species Reactivity:
Mouse
This product recognizes the mouse Thy1.2 alloantigen, expressed by thymocytes and peripheral T lymphocytes. This antibody reacts with Thy1.2 mice such as CBA and BALB/C, but not with Thy1.1 mice eg. AKR and FUB.
The antibody is particularly useful for removal of T lymphocytes from cell populations by complement mediated cytotoxicity, see Lake, P et al. for details.
This product is routinely tested in flow cytometry using mouse thymocytes.
Store at +4 °C or at -20 °C if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
Supplied as:
IgM fraction - liquid
Formulation:
Phosphate buffered saline
IgM fraction prepared by ammonium sulfate precipitation
This product
Spleen cells from immunised AKR mice were fused with cells of the mouse NS-1 myeloma cell line.
Expiration:
12 months from the date of shipment when stored properly.