Mouse Anti-Mouse TSC2 Antibody, 400 ul
$334.00
In stock
Code:
102-10150
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+

Product Description

Specifications

Size 400 µl
Protein Name / Synonyms TSC2
Accession Number P49815
Gene Symbols TSC2
Gene Id 7249
Concentration (lot specific) 0.5 mg/ml
Preparation This TSC2 monoclonal antibody is generated from mouse immunized with TSC2 recombinant protein.
Clonality Monoclonal
Species Mouse

Introduction

Mutations in this gene lead to tuberous sclerosis complex. Its gene product is believed to be a tumor suppressor and is able to stimulate specific GTPases. The protein associates with hamartin in a cytosolic complex, possibly acting as a chaperone for hamartin. Alternative splicing results in multiple transcript variants encoding different isoforms.

Specificity

Other Names
TSC2; TSC4; Tuberin; Tuberous sclerosis 2 protein
NCBI Accession #
NP_000539.2;NP_001070651.1;NP_001107854.1
Other Accession #
NP_000539.2; NP_001070651.1

Format

Type
Mouse Monoclonal Antibody (Mab)
Calculated Molecular Weight (Da)
200608
Recommended Dilutions
IF: 1:10-50; WB: 1:100
Fluorescent image of Hela cell stained with TSC2 Antibody(Cat# 102-10150/SG110509AA).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TSC2 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-mouse antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min).TSC2 immunoreactivity is localized to Microtubules significantly.
TSC2 Antibody (Cat# 102-10150) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the TSC2 antibody detected the TSC2 protein (arrow).

Antigen Source

HUMAN

Storage/Stability

2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.
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