Mouse Anti-Rat Nestin
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Anti-Rat Nestin Antibody
| Size | 0.1 mg |
| Estimated Lead Time | 1-2 weeks |
| Species | Rat |
| Specificity | This product recognizes nestin, a large intermediate filament protein originally identified by Hockfield and McKay (1985) It is predominately expressed in stem cells of the developing nervous system. Terminal differentiation is associated with a loss of nestin expression. Nestin expression has also been noted in other embryonic tissues, also in most Glioblastoma multiformes and many melanomas. |
| Host Species | Mouse |
| Protein Name / Synonyms | Nestin |
| Concentration (lot specific) | IgG concentration 0.5mg/ml |
| Recommended Applications | Immunohistology - Frozen, Immunofluorescence, Immunohistology - Paraffin, Western Blotting |
| Research Area | Neuroscience, Cardiovascular Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
This product reacts with a band at 200-220 kDa in reducing gels of newborn rat or mouse cell extracts.For the purpose of western blotting it is recommended that samples should be boiled in 4 volumes of 125 mM Tris, pH 6.8, 10% 2-mercaptoethanol, 10% glycerol and 4.6% SDS. Membranes should be blocked with milk or BSA. 5% PAGE gels are suggested.
Store at +4 °C or at -20 °C if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
If stored in this manner, this product is stable for 18 months after receipt.
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Anti-Rat Nestin Antibody