Mouse bFGF ELISA

RayBio® Mouse bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-bFGF-1
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Antigen Information

Accession Number:
  • P15655
Gene ID:
  • 14173
Gene Symbols:
  • FGF-2
  • FGF2
Protein Name & Synonyms:
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Target Species:
  • Mouse

Assay Format

Detection Range:
8 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma):
2 - 10 fold
Sensitivity:
8 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample type: Tissue Lysate
  2. Li Y., Wang W., Huang P., et al. Distinct sustained structural and functional effects of interleukin-33 and interleukin-25 on the airways in a murine asthma surrogate. Immunology. 2015 Aug;145(4):508-18. doi: 10.1111/imm.12465
    Species: Mouse
    Sample type: Tissue Lysate
  3. Okamoto K., Honda K., Doi K., et al. Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney. Am J Pathol. 2015 Jul;185(7):1889-98. doi: 10.1016/j.ajpath.2015.03.025
    Species: Mouse
    Sample type: Serum
  4. Akiyama M., et al. Trans-regulation of oligodendrocyte myelination by neurons through small GTPase Arf6-regulated secretion of fibroblast growth factor-2. Nature Communications.Published online August 21 2014. DOI: 10.1038/ncomms5744
    Species: Mouse
    Sample type: Conditioned Media
  5. Laschke MW. Et al., ADIPOSE TISSUE-DERIVED MICROVASCULAR FRAGMENTS FROM AGED DONORS EXHIBIT AN IMPAIRED VASCULARISATION CAPACITY. European Cells and Materials, Vol 28; 2014. 287-298.
    Species: Mouse
    Sample type: Conditioned Media
  6. Noda M., Takii., Parajuli B., Kawanokuchi J., Sonobe Y., et al FGF-2 released from degenerating neurons exerts microglial-induced neuroprotection via FGFR3-ERK signaling pathway. Journal of Neuroinflammation 2014, 11:76
    Species: Mouse
    Sample type: Conditioned Media
  7. Ozaki C, Somamoto S., Kawabata S., Tabata Y. Effect of an artificial silk elastin-like protein on the migration and collagen production of mouse fibroblasts. Journal of Biomaterials Science, 2014 Epub Ahead of Print, June 18 2014.DOI:10.1080/09205063.2014.926580
    Species: Mouse
    Sample type: Conditioned Media
  8. Cao X, Li L-P, Wang Q, et al. Astrocyte-derived ATP modulates depressive-like behaviors. Nat Med. 2013 Jun;19(6):773-777.
    Species: Mouse
    Sample type: Tissue Lysate
  9. Yan L. Dietary supplementation with curcumin enhances metastatic growth of Lewis lung carcinoma in mice. Int J Cancer. 2013 Jan 15;132(2):269-275.
    Species: Mouse
    Sample type: Plasma
  10. Kim, Bae Jin, et al. "Synergistic vasculogenic effects of AMD3100 and stromal-cell-derived factor-1? in vasa nervorum of the sciatic nerve of mice with diabetic peripheral neuropathy." Cell and tissue research 354.2 (2013): 395-407.
    Species: Mouse
    Sample type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.