RayBio® Mouse bFGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

8 pg/ml

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Detection Range
8 pg/ml - 600 pg/ml
Recommended Dilution (serum & plasma)
2 - 10 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data


This ELISA kit shows no cross-reactivity with following cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Protein Name & Synonyms
Fibroblast growth factor 2 (FGF-2) (Basic fibroblast growth factor) (bFGF) (Heparin-binding growth factor 2) (HBGF-2)
Accession Number
Gene Names
Fgf2, Fgf-2
Gene ID

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • FGF2

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Method of Detection:
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Huey K., Smith S., Sulaeman A., Breen E. Skeletal myofiber VEGF is necessary for myogenic and contractile adaptations to functional overload of the plantaris in adult mice. J Appl Physiol (1985). 2016 Jan 15;120(2):188-95. doi: 10.1152/japplphysiol.00638.2015.
    Species: Mouse
    Sample Type: Tissue Lysate

  2. Li Y., Wang W., Huang P., et al. Distinct sustained structural and functional effects of interleukin-33 and interleukin-25 on the airways in a murine asthma surrogate. Immunology. 2015 Aug;145(4):508-18. doi: 10.1111/imm.12465
    Species: Mouse
    Sample Type: Tissue Lysate

  3. Okamoto K., Honda K., Doi K., et al. Glypican-5 Increases Susceptibility to Nephrotic Damage in Diabetic Kidney. Am J Pathol. 2015 Jul;185(7):1889-98. doi: 10.1016/j.ajpath.2015.03.025
    Species: Mouse
    Sample Type: Serum

  4. Akiyama M., et al. Trans-regulation of oligodendrocyte myelination by neurons through small GTPase Arf6-regulated secretion of fibroblast growth factor-2. Nature Communications.Published online August 21 2014. DOI: 10.1038/ncomms5744
    Species: Mouse
    Sample Type: Conditioned Media

    Species: Mouse
    Sample Type: Conditioned Media

  6. Noda M., Takii., Parajuli B., Kawanokuchi J., Sonobe Y., et al FGF-2 released from degenerating neurons exerts microglial-induced neuroprotection via FGFR3-ERK signaling pathway. Journal of Neuroinflammation 2014, 11:76
    Species: Mouse
    Sample Type: Conditioned Media

  7. Ozaki C, Somamoto S., Kawabata S., Tabata Y. Effect of an artificial silk elastin-like protein on the migration and collagen production of mouse fibroblasts. Journal of Biomaterials Science, 2014 Epub Ahead of Print, June 18 2014.DOI:10.1080/09205063.2014.926580
    Species: Mouse
    Sample Type: Conditioned Media

  8. Cao X, Li L-P, Wang Q, et al. Astrocyte-derived ATP modulates depressive-like behaviors. Nat Med. 2013 Jun;19(6):773-777.
    Species: Mouse
    Sample Type: Tissue Lysate

  9. Yan L. Dietary supplementation with curcumin enhances metastatic growth of Lewis lung carcinoma in mice. Int J Cancer. 2013 Jan 15;132(2):269-275.
    Species: Mouse
    Sample Type: Plasma

  10. Laschke MW, Kleer S., Scheuer S., Farcia P., et al. VASCULARISATION OF POROUS SCAFFOLDS IS IMPROVED BY INCORPORATION OF ADIPOSE TISSUE-DERIVED MICROVASCULAR FRAGMENTS. European Cells and Materials, Vol 24, 2012 (pages 266-277)
    Species: Mouse
    Sample Type: Conditioned Media

  11. Yan L., DeMars LC. Dietary supplementation with methylseleninic acid, but not selenomethionine, reduces spontaneous metastasis of Lewis lung carcinoma in mice. Int J Cancer. 2012 Sep 15;131(6):1260-6. doi: 10.1002/ijc.27355
    Species: Mouse
    Sample Type: Plasma

  12. Talbot N., Sparks W., Powell A., et al. Quantitative and semiquantitative immunoassay of growth factors and cytokines in the conditioned medium of STO and CF-1 mouse feeder cells. In Vitro Cell Dev Biol Anim. 2012 Jan;48(1):1-11. doi: 10.1007/s11626-011-9467-7
    Species: Mouse
    Sample Type: Conditioned Media

  13. Tsubaki M., et al. Blockade of the Ras/MEK/ERK and Ras/PI3K/Akt pathways by statins reduces the expression of bFGF, HGF, and TGF-β as angiogenic factors in mouse osteosarcoma. Cytokine. 2011 Apr;54(1):100-7. doi: 10.1016/j.cyto.2011.01.005
    Species: Mouse

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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