Mouse Heat Shock Protein 70 ELISA Kit

RayBio® Mouse Heat Shock Protein 70 ELISA Kit.

Mouse Heat Shock Protein 70 ELISA Kit

RayBio® Mouse Heat Shock Protein 70 ELISA Kit.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	4-7 weeks
Species	Mouse
Specificity	The antibody pair provided in this kit recognizes Mouse Heat Shock Protein 70.
Accession Number	Q3U2G2
Gene Id	15525
Gene Symbols	HSPA4, MCG_113604
Protein Name / Synonyms	Heat shock 70 kDa protein 4 (Heat shock protein 4, isoform CRA_a)
Quantitative/Semi-Quantitative	Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Protein Folding
Shipping Type	Blue ice
Storage	≤-20°C
Note	This ELISA uses an antibody pair and standard that have been validated for multiplex array products, but not for the 96-well plate ELISA format. Thus, this ELISA requires a 4 - 7 week development phase, which RayBiotech begins after receiving an order for the kit. After development, the kit will have passed RayBiotech's ELISA quality control tests, and will thereafter have a typical 1 - 2 day lead time for orders placed.

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Customize This Mouse Heat Shock Protein 70 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified HSP70 (HSPA4), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (EL-PRELIM.pdf)

SDS (EL-PRELIM.pdf)

Excel (5af824478c7690acd2cf4518aeaae2058a44fa75.xlsm)

Product Features

Additional Notes

Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data

Application Notes

Kit Components

Pre-Coated 96-well Strip Microplate
Wash Buffer
Stop Solution
Assay Diluent(s)
Lyophilized Standard
Biotinylated Detection Antibody
Streptavidin-Conjugated HRP
TMB One-Step Substrate

Other Materials Required

Distilled or deionized water
Precision pipettes to deliver 2 µl to 1 µl volumes
Adjustable 1-25 µl pipettes for reagent preparation
100 µl and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
Log-log graph paper or computer and software for ELISA data analysis

Protocol Outline

Prepare all reagents, samples and standards as instructed in the manual.
Add 100 µl of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4°C.
Add 100 µl of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 µl of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

Customize This Mouse Heat Shock Protein 70 ELISA

We manufacture our ELISAs in-house, so we can build a custom version for your study.

Post-translational modifications (PTMs): Detect modified HSP70 (HSPA4), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
Also available: More sensitive platforms such as Simoa^® and NexaTag^®, multiplex panels, bulk lots, and other species when suitable reagents exist

Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.

Learn more about custom ELISA development

Featured Products

Mouse Heat Shock Protein 70 ELISA Kit

Mouse Heat Shock Protein 70 ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Customize This Mouse Heat Shock Protein 70 ELISA

Have this product in hand?

Featured Products

Mouse Heat Shock Protein 70 ELISA KitMouse Heat Shock Protein 70 ELISA Kit

Mouse Heat Shock Protein 70 ELISA KitMouse Heat Shock Protein 70 ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Customize This Mouse Heat Shock Protein 70 ELISA

Have this product in hand?

Featured Products

Mouse Heat Shock Protein 70 ELISA Kit

Mouse Heat Shock Protein 70 ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Customize This Mouse Heat Shock Protein 70 ELISA

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Mouse Heat Shock Protein 70 ELISA KitMouse Heat Shock Protein 70 ELISA Kit

Mouse Heat Shock Protein 70 ELISA KitMouse Heat Shock Protein 70 ELISA Kit

Product Description

Specifications

Product Features

Application Notes

Storage/Stability

Customize This Mouse Heat Shock Protein 70 ELISA

Documents

Have this product in hand?

Reviews

Ask A Question