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Mouse IL-1 beta ELISA

RayBio® Mouse IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, serum, and lysate samples.

$402.00  
ELM-IL1b-1
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Antigen Information

Accession Number:
  • P10749
Gene ID:
  • 16176
Gene Symbols:
  • IL1B
Protein Name & Synonyms:
Interleukin-1 beta (IL-1 beta)
Target Species:
  • Mouse

Assay Format

Detection Range:
5 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
5 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Wang P., Ji Y., Zhang X., et al. Targeting CASP8 and FADD-like apoptosis regulator ameliorates nonalcoholic steatohepatitis in mice and nonhuman primates. Nat Med. 2017 Apr;23(4):439-449. doi: 10.1038/nm.4290.
    Species: Mouse
    Sample type: Serum
  2. Jia Y., Li Z., Cai W., et al. SIRT1 regulates inflammation response of macrophages in sepsis mediated by long noncoding RNA. Biochim Biophys Acta Mol Basis Dis. 2018 Mar;1864(3):784-792. doi: 10.1016/j.bbadis.2017.12.029.
    Species: Mouse
    Sample type: Conditioned Media
  3. Garg, S., et al. "Intervention of probiotic L. reuteri fermented milk as an adjuvant to combat protein energy malnourishment induced gut disturbances in albino mice."?Journal of Functional Foods?36 (2017): 467-479.
    Species: Mouse
    Sample type: serum
  4. Othman, Azza I., et al. "Epigallocatechin-3-gallate protects against diabetic cardiomyopathy through modulating the cardiometabolic risk factors, oxidative stress, inflammation, cell death and fibrosis in streptozotocin-nicotinamide-induced diabetic rats."?Biomedicine & Pharmacotherapy?94 (2017): 362-373.
    Species: Rat
    Sample type: serum
  5. Nandi, Ajeya, and Biswadev Bishayi. "A novel CCR-2/TLR-2 triggered signaling in murine peritoneal macrophages intensifies bacterial (Staphylococcus aureus) killing by reactive oxygen species through TNF-R1."?Immunology letters?190 (2017): 93-107.
    Species: Mouse
    Sample type: Cell lysates
  6. Weisser M., Demel U., Stein S., et al. Hyperinflammation in patients with chronic granulomatous disease leads to impairment of hematopoietic stem cell functions. J Allergy Clin Immunol. 2016 Feb 4. pii: S0091-6749(16)00031-2. doi: 10.1016/j.jaci.2015.11.028
    Species: Mouse
    Sample type: Bone Marrow Aspirate
  7. Nouri H., Karkhah A., Mohammadzadeh I., Sankian M. Elevated caspase?1 activity and IL?1? expression are associated with the IPAF inflammasome in an experimental model of allergy. Mol Med Rep. 2016 Apr;13(4):3356-62. doi: 10.3892/mmr.2016.4934.
    Species: Mouse
    Sample type: Tissue Lysate
  8. Thomas J., Khanam R., Vohora D. Augmentation of effect of venlafaxine by folic acid in behavioral paradigms of depression in mice: Evidence of serotonergic and pro-inflammatory cytokine pathways. Pharmacol Rep. 2016 Apr;68(2):396-403. doi: 10.1016/j.pharep.2015.10.003.
    Species: Mouse
    Sample type: Serum
  9. Zhang S., Wang Y., Li D., et al. Necrostatin-1 Attenuates Inflammatory Response and Improves Cognitive Function in Chronic Ischemic Stroke Mice. Medicines 2016, 3(3), 16; doi:10.3390/medicines3030016
    Species: Mouse
    Sample type: Tissue Lysate
  10. Yu J., Yuan X., Liu Y., et al. Delayed Administration of WP1066, an STAT3 Inhibitor, Ameliorates Radiation-Induced Lung Injury in Mice. Lung (2016) 194: 67. doi:10.1007/s00408-015-9821-8
    Species: Mouse
    Sample type: Serum
Used cell culture sample type. It is not as costly as other kits but requires more labor. Results will be as consistent as the user's technique. If you have previous experience with ELISA you will have no problems, if you are new to ELISA it may be more difficult.

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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