Mouse IL-13 ELISA

$398.00
ELM-IL13-1

RayBio® Mouse IL-13 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
6 pg/ml

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Detection Range
6 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, TPO, VEGF.
Species Detected
Mouse
Protein Name & Synonyms
Interleukin-13 (IL-13) (T-cell activation protein P600)
Accession Number
Gene Names
Il13, Il-13
Gene ID
16163

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL13

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Gupta S, Duraiswamy B, Muthureddy Nataraj SK, Rama SatyanarayanaRaju K, Babu UV, et al. (2014) Inhibitory Potential of Yucca Gloriosa L. Extractand Isolated Gloriosaol Isomeric Mixture on Ovalbumin Induced AirwayHyperresponsiveness in Balb/C Mice. Clinic Pharmacol Biopharmaceut S2: 002.doi:10.4172/2167-065X.S2-002
    Species: Mouse
    Sample Type: BAL Fluid

  2. Gupta S., Duraiswamy B., Nataraj S., Raju R., Uv B., et al. Inhibitory Potential of Yucca Gloriosa L . Extract and Isolated Gloriosaol Isomeric Mixture on Ovalbumin Induced Airway Hyperresponsiveness in Balb/C Mice. Clinic Pharmacol Biopharmaceut 2014, S2 http://dx.doi.org/10.4172/2167-065X.S2-002
    Species: Mouse
    Sample Type: BAL Fluid

  3. Rama Satyanarayana Raju K, 5-Aminosalicylic acid attenuates allergeninduced airway inflammation and oxidative stress in asthma, Pulmonary Pharmacology & Therapeutics (2014), doi: 10.1016/j.pupt.2014.07.007.
    Species: Mouse
    Sample Type: BAL Fluid

  4. Chung M., Park J., Park Y. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int Immunopharmacol. 2012 Feb;12(2):453-9. doi: 10.1016/j.intimp.2011.12.027
    Species: Mouse
    Sample Type: Conditioned Media

  5. Chung M., Park J., Park Y. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int Immunopharmacol. 2012 Feb;12(2):453-9. doi: 10.1016/j.intimp.2011.12.027
    Species: Mouse
    Sample Type: BAL Fluid

  6. Chung M., Park J., Park Y. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int Immunopharmacol. 2012 Feb;12(2):453-9. doi: 10.1016/j.intimp.2011.12.027
    Species: Mouse
    Sample Type: Serum

  7. Chung M., Park J., Park Y. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int Immunopharmacol. 2012 Feb;12(2):453-9. doi: 10.1016/j.intimp.2011.12.027
    Species: Mouse
    Sample Type: Tissue Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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