Mouse IL-13 ELISA

RayBio® Mouse IL-13 ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P20109
Gene ID:
  • 16163
Gene Symbols:
  • IL-13
  • IL13
Protein Name & Synonyms:
Interleukin-13 (IL-13) (T-cell activation protein P600)
Target Species:
  • Mouse

Assay Format

Detection Range:
6 pg/ml - 2000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
6 pg/ml

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This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse 6Ckine, CTACK, Eotaxin, GCSF, GM-CSF, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p40, IL-12p70, IL-17, IFN-gamma, KC, Leptin, MCP-5, MIP-1 alpha, MIP-2, MIP-3 beta, RANTES, SCF, sTNFri, TARC, TIMP-1, TNF-alpha, TPO, VEGF.
Number of Targets Detected:
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Gupta S, Duraiswamy B, Muthureddy Nataraj SK, Rama SatyanarayanaRaju K, Babu UV, et al. (2014) Inhibitory Potential of Yucca Gloriosa L. Extractand Isolated Gloriosaol Isomeric Mixture on Ovalbumin Induced AirwayHyperresponsiveness in Balb/C Mice. Clinic Pharmacol Biopharmaceut S2: 002.doi:10.4172/2167-065X.S2-002
    Species: Mouse
    Sample type: BAL Fluid
  2. Gupta S., Duraiswamy B., Nataraj S., Raju R., Uv B., et al. Inhibitory Potential of Yucca Gloriosa L . Extract and Isolated Gloriosaol Isomeric Mixture on Ovalbumin Induced Airway Hyperresponsiveness in Balb/C Mice. Clinic Pharmacol Biopharmaceut 2014, S2
    Species: Mouse
    Sample type: BAL Fluid
  3. Rama Satyanarayana Raju K, 5-Aminosalicylic acid attenuates allergeninduced airway inflammation and oxidative stress in asthma, Pulmonary Pharmacology & Therapeutics (2014), doi: 10.1016/j.pupt.2014.07.007.
    Species: Mouse
    Sample type: BAL Fluid
  4. Chung M., Park J., Park Y. Anti-inflammatory effects of low-molecular weight chitosan oligosaccharides in IgE-antigen complex-stimulated RBL-2H3 cells and asthma model mice. Int Immunopharmacol. 2012 Feb;12(2):453-9. doi: 10.1016/j.intimp.2011.12.027
    Species: Mouse
    Sample type: Tissue Lysate
  5. Rogerio, Alexandre P., Edinéia L. Andrade, and João B. Calixto. "C-fibers, but not the transient potential receptor vanilloid 1 (TRPV1), play a role in experimental allergic airway inflammation." European journal of pharmacology 662.1 (2011): 55-62.
    Species: Mouse
    Sample type: BAL Fluid
This human IL-13 ELISA kit is simple and steps were clear. Just need to use different sample diluents for serum vs lysates. We used the kit with supernatant from mice BAL. The kit worked well and standard ran evenly, kit was able to detect a wide range of IL-13 levels. Would definitely order again!

University of Pittsburgh
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.