Mouse IL-2 ELISA

RayBio® Mouse IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

ELM-IL2-1
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Antigen Information

Accession Number:
  • P04351
Gene ID:
  • 16183
Gene Symbols:
  • IL-2
  • IL2
Protein Name & Synonyms:
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Target Species:
  • Mouse

Assay Format

Detection Range:
1 pg/ml - 40 pg/ml
Recommended Dilution (serum & plasma):
2 fold
Sensitivity:
1 pg/ml

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Specificity:
This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Number of Targets Detected:
1
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Colorimetric
Quantitative/Semi-Quantitative:
Quantitative
Solid Support:
96-well Microplate

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Dey P., Ray S., Chaudhuri T. Immunomodulatory activities and phytochemical characterisation of the methanolic extract of Dioscorea alata aerial tuber. Journal of Functional Foods . Volume 23, May 2016, Pages 315-328. https://doi.org/10.1016/j.jff.2016.02.044
    Species: Mouse
    Sample type: Conditioned Media
  2. Dey P., Chaudhuri TK. Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/T H2 cytokine balance in murine splenic lymphocytes. Cytotechnology. 2015 Feb 14. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  3. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample type: Serum
  4. Li W., Li M., Deng G., et al. Prime-boost vaccination with Bacillus Calmette Guerin and a recombinant adenovirus co-expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. Mol Med Rep. 2015 Aug;12(2):3073-80. doi: 10.3892/mmr.2015.3770
    Species: Mice
    Sample type: Conditioned Media
  5. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-InducedAcute Lung Inflammation Through Inhibition of NF-?b, STAT3 and Pro-Inflammatory Mediators and Enhancement of I?B? and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y
    Species: Mouse
    Sample type: Pleural Fluid
  6. Gu X., Zie Y., Wang S., Peng X., Lai S., Yang G. Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice. Exp Appl Acarol. 2014 Apr 13. [Epub ahead of print]
    Species: Mouse
    Sample type: Conditioned Media
  7. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v
    Species: Mouse
    Sample type: Serum
  8. Tian X., et al. In Vivo Immunotoxicity of SiO2@(Y0.5Gd0.45Eu0.05)2O3 as Dual-Modality Nanoprobes. Int. J. Mol. Sci. 2014, 15, 13649-13662; doi:10.3390/ijms150813649
    Species: Mouse
    Sample type: Serum
  9. Tian X., et al. Toxicity evaluation of Gd2O3@SiO2 nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo. International Journal of Nanomedicine. 2014 Aug 21;9:4043-53. doi: 10.2147/IJN.S66164.
    Species: Mouse
    Sample type: Serum
  10. Armada, Ana, et al. "Generation of an antibody that recognizes Plasmodium chabaudi cysteine protease (chabaupain-1) in both sexual and asexual parasite life cycle and evaluation of chabaupain-1 vaccine potential." Experimental parasitology 135.1 (2013): 166-174.
    Species: Mouse
    Sample type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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