Mouse IL-2 ELISA

$398.00
ELM-IL2-1

RayBio® Mouse IL-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
1 pg/ml

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Detection Range
1 pg/ml - 40 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN-gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-2, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF.
Species Detected
Mouse
Protein Name & Synonyms
Interleukin-2 (IL-2) (T-cell growth factor) (TCGF)
Accession Number
Gene Names
Il2, Il-2
Gene ID
16183

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL2

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Mouse
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Dey P., Chaudhuri TK. Immunomodulatory activity of Nerium indicum through inhibition of nitric oxide and cyclooxygenase activity and modulation of TH1/T H2 cytokine balance in murine splenic lymphocytes. Cytotechnology. 2015 Feb 14. [Epub ahead of print]
    Species: Mouse
    Sample Type: Conditioned Media

  2. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample Type: Conditioned Media

  3. Roy S., Chaudhuri T. Assessment of Th1 and Th2 cytokine modulatory activity of an edible fern, Diplazium esculentum. Food and Agricultural Immunology Volume 26, Issue 5, 2015 . DOI: 10.1080/09540105.2015.1007449
    Species: Mouse
    Sample Type: Serum

  4. Li W., Li M., Deng G., et al. Prime-boost vaccination with Bacillus Calmette Guerin and a recombinant adenovirus co-expressing CFP10, ESAT6, Ag85A and Ag85B of Mycobacterium tuberculosis induces robust antigen-specific immune responses in mice. Mol Med Rep. 2015 Aug;12(2):3073-80. doi: 10.3892/mmr.2015.3770
    Species: Mice
    Sample Type: Conditioned Media

  5. Ahmad SF., et al. Naringin Attenuates the Development of Carrageenan-InducedAcute Lung Inflammation Through Inhibition of NF-κ, STAT3 and Pro-Inflammatory Mediators and Enhancement of IκBα and Anti-Inflammatory Cytokines. Inflammation, 2014 Online first view August, DOI: 10.1007/s10753-014-9994-y
    Species: Mouse
    Sample Type: Pleural Fluid

  6. Gu X., Zie Y., Wang S., Peng X., Lai S., Yang G. Immune response induced by candidate Sarcoptes scabiei var. cuniculi DNA vaccine encoding paramyosin in mice. Exp Appl Acarol. 2014 Apr 13. [Epub ahead of print]
    Species: Mouse
    Sample Type: Conditioned Media

  7. Liu J., et al. Sub-10 nm Monoclinic Gd2O3:Eu3+ Nanoparticles as Dual-Modal Nanoprobes for Magnetic Resonance and Fluorescence Imaging. Langmuir, Article ASAP, 2014. DOI: 10.1021/la503228v
    Species: Mouse
    Sample Type: Serum

  8. Tian X., et al. In Vivo Immunotoxicity of SiO2@(Y0.5Gd0.45Eu0.05)2O3 as Dual-Modality Nanoprobes. Int. J. Mol. Sci. 2014, 15, 13649-13662; doi:10.3390/ijms150813649
    Species: Mouse
    Sample Type: Serum

  9. Tian X., et al. Toxicity evaluation of Gd2O3@SiO2 nanoparticles prepared by laser ablation in liquid as MRI contrast agents in vivo. International Journal of Nanomedicine. 2014 Aug 21;9:4043-53. doi: 10.2147/IJN.S66164.
    Species: Mouse
    Sample Type: Serum

  10. Bizargity P, Liu K, Wang L, Hancock WW, Visner GA. Inhibitory Effects of Pirfenidone on Dendritic Cells and Lung Allograft Rejection. Transplantation. 2012;94(2):114-122. doi:10.1097/TP.0b013e3182584879.
    Species: Mouse
    Sample Type: Cell Lysate

  11. Rodrigues MA., Figueiredo L., Padrela L., et al. Development of a novel mucosal vaccine against strangles by supercritical enhanced atomization spray-drying of Streptococcus equi extracts and evaluation in a mouse model. Eur J Pharm Biopharm. 2012 Oct;82(2):392-400. doi: 10.1016/j.ejpb.2012.07.005.
    Species: Mouse
    Sample Type: Conditioned Media

  12. Figueiredo L., Cadete A., Goncalves L., et al. Intranasal immunisation of mice against Streptococcus equi using positively charged nanoparticulate carrier systems. Vaccine. 2012 Oct 12;30(46):6551-8. doi: 10.1016/j.vaccine.2012.08.050.
    Species: Mouse
    Sample Type: Conditioned Media

  13. Sun A, Wei H, Sun R, Xiao W, Yang Y, Tian Z. Human Interleukin-15 Improves Engraftment of Human T Cells in NOD-SCID mice. Clin Vaccine Immunol. 2006;13(2):227-234.
    Species: Mouse
    Sample Type: Conditioned Media

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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