Detects 8 phosphorylated proteins in mouse samples. Suitable for all liquid sample types but intended for use with cell and tissue lysates.
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Product Description
Specifications
| Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Mouse |
| Compatible Sample Types | Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 8 |
| Recommended Applications | Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process |
| Solid Support | Membrane |
| Design Principle | Sandwich-based |
| Method Of Detection | Chemiluminescence |
| Research Area | Post-Translational Modifications, Phosphorylation, TGF-beta Signaling, PKC Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Easy to use
- No specialized equipment needed
- Compatible with nearly any liquid sample
- Proven technology (many publications)
- Highly sensitive (pg/ml)
- Sandwich ELISA specificity
- Higher density than ELISA, Western blot or bead-based multiplex
Target Names
c-Jun (P-Ser73), SMAD1 (P-Ser463/465), SMAD5 (P-Ser463/465), SMAD4 (P-Thr277), ATF2 (P-Thr69/71), C-Fos (P-Thr232), SMAD2 (P-Ser245/250/255), TAK1 (P-Ser412)
Kit Components
- Antibody Arrays
- Blocking Buffer
- Detection Antibody Cocktail
- HRP-Anti-Rabbit IgG Concentrate
- Wash Buffer I Concentrate
- Wash Buffer II Concentrate
- Cell Lysis Buffer Concentrate
- Detection Buffer C
- Detection Buffer D
- 8-Well Incubation Tray w/ Lid
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail I
- Phosphatase Inhibitor Cocktail II
- Plastic Sheets
- Array Map Template
Additional Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or plastic wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system
Assay Procedure Summary
- Block membranes
- Incubate with Sample
- Incubate with Detection Antibody Cocktail
- Incubate with HRP-Conjugated anti-IgG
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Typical Data
Storage/Stability
Store kit at ≤ -20°C immediately upon arrival. Kit must be used before the 6 month expiration date.
