Introduction
RayBiotech is committed to develop a series of phosphorylation antibody arrays. RayBio® Mouse Protein Tyrosine Phosphorylation Antibody Array 2 is specifically designed for simultaneous identification of the relative levels of phosphorylation of 500 different mouse proteins in cell lysate. By monitoring the changes in protein tyrosine phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.
To use the G-Series Mouse Protein Tyrosine Phosphorylation Antibody Array 2, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and biotinylated anti-phosphotyrosine antibodies are then used to detect the phosphorylated tyrosines on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
14-3-3 beta, 14-3-3 zeta, 53BP1, aAmylase, AAT1, ABAT, ABCF1 , ABI3BP, ACAA1, ACAA2, ACACA, ACLY, ACO1, ACTBL2, ACTC1, ACTG1, ACTG2, ACTN1, ADA, ADAMDEC1 , ADAS, ADGRF5, ADGRL4, ADH1, ADH1C, ADH4 , ADH5, ADM, ADP-Ribosyl Cyclase 2, Advillin, AEBP1, AFG3L2 , AGA, Aggrecan, Agrin, AGXT, Ahsp , AIFM1, AKAP9 , AKR1B1, AKR7A2 , ALAD, ALDH16A1 , ALDH1A1, ALDH9A1 , Alkaline Phosphatase, alpha Actinin 4, alpha Tubulin 4, Alpha-synuclein, ALS, Alsin , Aminoacylase 1, Aminopeptidase A, Androgen Receptor, ANGPTL6 , ANGPTL8, Ankrd26, Annexin A1, Annexin A2, Annexin A5, Annexin A6, ANP, ANP32A, Antithrombin III, APLP1, AQR, ARFGEF3, Arp3 , ARPC2, ARPC3, ARPP19, ART3 , ARTS1, ASGR2, ASH2L , ASL, Aspartate Aminotransferase, Aspartyl Aminopeptidase, ASXL1 , ATP5A1, ATPB, B3GNT2, B4GalT1, B7-H2, bad, BASP1, Bassoon , bcl-w, BCoR , beta I Spectrin , beta I Tubulin, beta III Tubulin, BID, BIN2, Biotinidase, BIRC6 , BMP-1, BPGM , BPIFB1, BPIFB2 , Brevican, BRG1, BRSK1, C1QA, C1QB , C1QR, C1RL, C1S, C4BPA , C6, C8A, C8G, C9orf40, CA1, CA150 , CACNB4, Cadherin-22 , Cadherin-6, CALD1, Calpain S1 , Calpastatin, Calponin-2, Calretinin, Calumenin , CAP1 , CAPZA1 , Carbonic Anhydrase II, Carbonic Anhydrase III, Carboxylesterase 1, Carboxypeptidase M, Carboxypeptidase E, Caspase-14, Catalase, Cathelicidin, Cathepsin A, Cathepsin G , Cathepsin H, Cathepsin Z, CBS , CCAR2, CCDC126 , CCDC25, CCS, CD109, CD133, CD148, CD155, CD21, CD39L4, CD41, CD42b, CD48, CD5L, CD98, CDA, CDK2 , CED-6, CENPF, CEP57 , Cezanne , CFB, CFHR1, CFI, CFVII, Chitobiase, Chitotriosidase(1), Cholinesterase, CHORDC1 , CHREBP, Chromogranin B, CKB, CLIC1, CLIP1, CL-P1, CLTA, CNOT1, CO4A2, Coagulation Factor IX, Coagulation Factor V , Coagulation Factor XI, Coagulation Factor XII , Coagulation Factor XIII, Cofilin-1, COG4 , COL19A1 , COL4A3 , Col6A2, COL9A3 , COLEC10 , Collagen I alpha-1, Collagen III , Collagen IV alpha-6 , Collagen IX , Collagen V, Collagen X , Collagen XV , Corneodesmosin, Cortactin, COTL1 , CPB2, CPEB3 , CPNE3 , CRHBP, CrkL(1), CRMP2, CRTAC1, CRYZ, Cyclophilin A, Cyclophilin B, Cystatin, CYTL1, Cytochrome b5, Cytochrome C, Cytokeratin 1, Cytokeratin 10, Cytokeratin 13, Cytokeratin 14, Cytokeratin 15, Cytokeratin 20 , Cytokeratin 9 , D4, DAN, DARS2 , DBH, DCXR, DDAH1, DDT, DDX3Y, DEFA6, Desmocollin 1, Desmocollin-2, Desmocollin-3, Desmoglein-1, Desmoglein-2, Desmoplakin 3, DGK-theta, DISC 1, DMRN9, DOT1L, DPP3, DRIL1 , DSCAM, DSPG3, ECHS1 , ECI1, ECM-1, EEF1G , EEF2, EFEMP2 , EFTUD2, EHD3 , Eif4a1, ELAVL1 , EMSY , EN2 , Endorepellin, ENO3, ENSA , EPB41, EPCR, Ephrin-B1, Eps 15, ERAB, ERp29, ERp57, ERp72, ESD, ESR1, Ezrin, FABP5, FAH, FAM20C , FAM3C, FASN, FASTKD5, FBP 38 , FDPS , FGG, Fibrillin 1, Fibrinogen-like 2, Fibrinopeptide B, Fibulin 3, Ficolin 2, Filamin C, FKBP1A, FKBP25, FKBP51, Fodrin alpha chain, Frizzled 8 , FRY, FSH-B, FTL1, FUCA2, FUS , G3BP1, G6PD, GALNT2, GANAB, GAPHD, GARNL1 , GART, Gastrokine 1, GATM, GBE1, GCDFP 15, GCLC , GCSH , GDA, GDF7 , GDI1, GDI2, Gephyrin, GFAP, GGCT, GGH, GIP, GLIPR2, GLUD1 , Glycoprotein V, GM2A, GMF beta, GNB1 , GNPTG, GOLIM4, GOLM1, GPD1, GPLD1, GRHPR, GRP170, GSS, GSTM1, GSTO1, GSTP1, Guanylin, GZMM, H6PD, HABP2, HBB, HDGF, Hemoglobin, Hemoglobin A1c, HEXB, HGFA, HIBADH , HINT1, HIP1R , Histone H1.2 , Histone H1.4, Histone H2A , Histone H2A.Z, Histone H2B K, Histone H3.3 , Histone H4 , HMGB1, HMGB2 , HMGB3, HMGN2, HNF-3 alpha, hnRNP A1 , hnRNP A2B1 , hnRNP C1 + C2 , hnRNP G , hnRNP L , hnRNP M , hnRNP U , Hornerin, Hoxb3 , HOXD11 , HP1BP3, HPD, HPRG, HPRT1, HRP12, HSPA1A, HTRA1, HUWE1, IDH1, IFRD1 , IGFBP-rp1, IGSF4B, ILK, IMP2/IGF2BP2/p62, Inhibin beta , Integrin alpha-6, Integrin b1, Integrin beta-6, IQGAP2 , IRE1, IRS2 , ISOC2, ITGB4BP, ITIH2, ITIH3, ITIHC4, JAM-A, JPT1, KDM4B, Keratin 36, KIAA0319L, KIAA1468, KLKB1, KMT2D, KRT31, KRT33B, KRT73, KRT82, KRT85 - N-terminal, KSR1 , LAF4, LAIR1, LAM b1, Lamin A/C, Lamin B2 , Laminin 2 alpha, Laminin b2 , Laminin gamma 1, LAMP, LAMP1, LASP1 , LCAT , LCMT2 , LDH-H, LEDGF, Limbin, LIMS1, LMW-PTP, LOK , LOX , LOXL1, LPA, LTBP4, Lubricin, LUZP1 , LYZL1, MAGI2, MAN1 , MAN1A1, Mannosidase II , MAP1A , MAPRE1 , MARCKS, MASP3, MBD2 , MBP, MCAM, Mcl-1, MCM, MDH1 , Meprin alpha, Metallothionein 2, Metavinculin, MFAP4, MFI2, mGLUR5, Mimecan, MLCK, MMR, MN1 , Moesin, MP1, MPCA, MRP 1, MSH6, Mtor, Multimerin 2, MyBPC3, Myeloperoxidase, MYH2 , MYH6, MYH7, MYHC 2x, MYL12B , MYO5A , Myoferlin , Myosin 18B , Myosin9, NABC1 , NAGLU, NAP1L1 , NAPRT1, NASP , NCAM2 , Nebulin , Nectin-1, Nectin-3, Neogenin, Nesprin2, Neurofibromin , Neurogranin, Neuropeptide B, Neuropilin-1, Neurotrimin, NF-M, NIF3L1, NME3 , nNOS1, Notch-2, NPAS3 , NPM1, Thrombospondin 5
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Tyrosine Phosphorylation Antibody Array G2 Slide(s)
- 2X Cell Lysis Buffer
- Protease Inhibitor Cocktail
- Phosphatase Inhibitor Cocktail Set II
- Blocking Buffer
- 20X Wash Buffer I
- 20X Wash Buffer II
- Biotin-Conjugated Anti-phosphotyrosine
- Fluorescent dye-Conjugated Streptavidin
- Wash Buffer III
- Adhesive film
Other Materials Required
- Shaker
- Laser scanner for fluorescence detection
- Aluminum foil
- Distilled water
- Plastic box
- 50 ml Centrifuge tube
- Isopropanol (2-propanol)
Protocol Outline
- Dry the Glass Slide
- Blocking and Incubation
- Fluorescence Detection
Storage/Stability
Upon receipt, the kit should be stored at -20°C to -80°C. Please use within 6 months from the date of shipment. After initial use, the 2X Cell Lysis Buffer, Blocking Buffer, 20X Wash Buffer I, 20X Wash Buffer II, Biotin-Conjugated Anti-phosphotyrosine and Fluorescent dye-Conjugated Streptavidin (Cy3 equivalent) should be stored at 4°C to avoid repeated freeze-thaw cycles. The Array I Glass Slide, Protease Inhibitor Cocktail and Phosphatase Inhibitor Cocktail Set II should be kept at -20°C to -80°C. Use within 3 months after initial use.