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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 3-5 business days |
| Species | Human |
| Sensitivity | 30 pg/mL |
| Detection Range | 137.17 pg/mL - 100000 pg/mL |
| Specificity | The antibodies used in the kit are specific to its own protein. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2- to 10-fold |
| Accession Number | P05067 |
| Gene Id | 351 |
| Gene Symbols | APP, A4, AD1 |
| Protein Name / Synonyms | Amyloid-beta precursor protein (APP) (ABPP) (APPI) (Alzheimer disease amyloid A4 protein homolog) (Alzheimer disease amyloid protein) (Amyloid precursor protein) (Amyloid-beta (A4) precursor protein) (Amyloid-beta A4 protein) (Cerebral vascular amyloid peptide) (CVAP) (PreA4) (Protease nexin-II) (PN-II) [Cleaved into: N-APP; Soluble APP-alpha (S-APP-alpha); Soluble APP-beta (S-APP-beta); C99 (Beta-secretase C-terminal fragment) (Beta-CTF); Amyloid-beta protein 42 (Abeta42) (Beta-APP42); Amyloid-beta protein 40 (Abeta40) (Beta-APP40); C83 (Alpha-secretase C-terminal fragment) (Alpha-CTF); P3(42); P3(40); C80; Gamma-secretase C-terminal fragment 59 (Amyloid intracellular domain 59) (AICD-59) (AID(59)) (Gamma-CTF(59)); Gamma-secretase C-terminal fragment 57 (Amyloid intracellular domain 57) (AICD-57) (AID(57)) (Gamma-CTF(57)); Gamma-secretase C-terminal fragment 50 (Amyloid intracellular domain 50) (AICD-50) (AID(50)) (Gamma-CTF(50)); C31] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | qPCR |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Ultra-sensitivity: Up to 1,000x more sensitive than traditional ELISA
- Ultra-low sample volume: Less than 10 µL needed
- Specificity: Sandwich-based detection
- Simplicity: Two steps processing
- Efficiency: Get data within 4 hours
- Multiplexing ready*
*Increase your throughput with RayBiotech's customizable multiplex bead-based immuno-PCR kits, designed for panels of up to 5 targets. This platform helps you save time, reduce costs, and decrease sample consumption by >2000% compared to traditional colorimetric ELISA. For more details, reach out to us at techsupport@raybiotech.com.
Standard Curve
Spiking & Recovery
| Sample Type | Recovery (%) | Range (%) |
|---|---|---|
| Serum | 93 | 85-101.0 |
| Cell Culture Supernatants | 54 | 47-71 |
Kit Components
| Component | Size / Qty |
|---|---|
| 96-well V-bottom Plate | 1 plate |
| 96-well Plate Film | 1 piece |
| 96-well PCR Plate | 1 plate |
| 1X Assay Diluent | 1 bottle |
| 20X Wash Buffer Concentrate | 1 bottle |
| Lyophilized Standard | 1 vial |
| Antibody Coated Beads | 1 vial |
| Oligonucleotide Conjugated Antibody | 1 vial |
| 1X Elution Buffer | 1 vial |
| 2X PCR Master Mix | 1 vial |
| Primer Mix | 1 vial |
| PCR Plate Film | 1 piece |
*The PCR plate used is a 0.2 mL, non-skirted 96-well plate (Thermo Fisher, cat. no. AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (techsupport@raybiotech.com).
Additional Materials Required
- Real-time PCR instrument, Bio-Rad or QuantStudio™ recommended
- Orbital 96-well plate shaker (with ability to reach 700 rpm)
- RayBio® 96-well Magnetic Plate Separator (Catalog #: BIQ-MPS) or similar
- Optional: Automated microplate washer (BioTek 405 TS Washer recommended) with magnetic separation (RayBio® 96-well Magnetic Plate Separator recommended)
- Precision pipettes to deliver 1 μL to 1 mL volumes
- Pipette tips (barrier tips are recommended to reduce contamination)
- Adjustable 1-25 mL pipettes for reagent preparation
- Centrifuge that can spin 96 well plates at 500 g
- Nuclease-free water or deionized water
- 1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
- Absorbent paper
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add 5 μL of the bead and conjugate mix, and 5 μL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
- Add 40 μL Assay Diluent and wash the plate using a magnetic plate separator or automated microplate washer.
- Add 40 μL Elution buffer to each well. Incubate for 10 minutes at room temperature.
- Prepare PCR/Primer Master Mix and pipette 15 μL into each well of the PCR plate.
- Transfer 5 μL supernatant from V-bottom plate to the PCR plate.
- Run real-time PCR.
Storage/Stability
Upon receiving the kit, store 2X PCR Master Mix, Primer Mix and Lyophilized Standard at -20°C. Store other components at 4°C. If stored at the proper temperature, the kit may be stored for up to 12 months from the date of shipment. Avoid repeated freeze-thaw cycles.