25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

25% off all ELISA kits

Use code ELISA25-262 for 25% off all ELISAs through November.

NexaTag^® Human Inflammation qIPCR Multiplex ELISA Array 4

The NexaTag qIPCR Multiplex ELISA enables simultaneous quantification of multiple proteins in serum, plasma, cell culture supernatants, and cell lysates, while maintaining the ultrasensitivity characteristic of singleplex NexaTag assays.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	3-5 business days
Species	Human
Specificity	The antibodies included in the kit are specific for their respective target proteins.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates
Recommended Dilution (Serum/Plasma)	2- to 10-fold
Accession Number	P48023, Q8WWZ1, Q13007, P08700, O95760
Gene Id	356, 84639, 11009, 3562, 90865
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	5
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	qPCR
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Ultra-sensitivity: Up to 1,000x more sensitive than traditional ELISA
Ultra-low sample volume: Less than 10 µL needed
Specificity: Sandwich-based detection
Simplicity: Two steps processing
Efficiency: Get data within 4 hours
Multiplex: Simultaneous quantification of 5 biomarkers

Target Names

Scroll over each target protein for more information
Fas L Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6 Gene ID: 356 Accession #: P48023 ELISA Kit: NexaTag® Human FasL qIPCR ELISA Kit	IL-1 F10 Gene Symbol: IL1F10 FIL1T IL1HY2 IL38 FKSG75 UNQ6119/PRO20041 Gene ID: 84639 Accession #: Q8WWZ1 ELISA Kit: NexaTag® Human IL-1F10 qIPCR ELISA Kit	IL-24 Gene Symbol: IL24 MDA7 ST16 Gene ID: 11009 Accession #: Q13007 ELISA Kit: NexaTag® Human IL-24 qIPCR ELISA Kit	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: NexaTag® Human IL-3 qIPCR ELISA Kit	IL-33 Gene Symbol: IL33 C9orf26 IL1F11 NFHEV Gene ID: 90865 Accession #: O95760 ELISA Kit: NexaTag® Human IL-33 qIPCR ELISA Kit

Kit Components

Component	Size / Qty
96-well V-bottom Plate	1 plate
96-well Plate Film	1 piece
96-well PCR Plate*	1 plate
Assay Diluent	1 bottle
Wash Buffer	1 bottle
Lyophilized Standard Mix	1 vial
Antibody Coated Beads Mix	1 vial
Oligonucleotide Conjugated Antibody	1 vial
Elution Buffer	1 vial
PCR Master Mix	1 vial
Primer Mix	1 vial
TaqMan Probe Mix	1 vial
PCR Plate Film	1 piece

*The PCR plate used is a 0.2 mL, non-skirted 96-well plate (Thermo Fisher, cat. no. AB0600). Please ensure compatibility with your PCR machine prior to purchase. For additional information contact technical support (techsupport@raybiotech.com).

Additional Materials Required

Real-time PCR instrument capable of reading Real-time PCR instrument capable of detecting 5 channels in qPCR: FAM, VIC/HEX, ABY/TAMRA, JUN/ROX and Cy5 (Bio-Rad or QuantStudio™ recommended)
Orbital 96-well plate shaker (with ability to reach 700 rpm)
RayBio^® 96-well Magnetic Plate Separator (Catalog #: BIQ-MPS) or similar
Optional: Automated microplate washer (BioTek 405 TS Washer recommended) with magnetic separation (RayBio^® 96-well Magnetic Plate Separator recommended)
Precision pipettes to deliver 1 μL to 1 mL volumes
Pipette tips (barrier tips are recommended to reduce contamination)
Adjustable 1-25 mL pipettes for reagent preparation
Centrifuge that can spin 96 well plates at 500 g
Nuclease-free water or deionized water
1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
Absorbent paper

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 5 µL of the bead and conjugate mix, and 5 µL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
Add 40 µL Assay Diluent and wash the plate five times using a magnetic plate separator or automated microplate washer.
Add 40 µL Elution buffer to each well. Incubate for 10 minutes at room temperature.
Prepare TaqMan Probe/Primer/PCR Master Mix and pipette 15 µL into each well of the PCR plate.
Transfer 5 µL supernatant from V-bottom plate to the PCR plate.
Run real-time PCR.

Storage/Stability

Upon receiving the kit, store PCR Master Mix, TaqMan Probe Mix, Primer Mix, and Antigen Standard Mix at -20°C. Store other components at 4°C. If stored at the proper temperature, the kit may be stored for up to 12 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (MIQH-INF4.pdf)

SDS (MIQH-INF4.pdf)

Product Features

Ultra-sensitivity: Up to 1,000x more sensitive than traditional ELISA
Ultra-low sample volume: Less than 10 µL needed
Specificity: Sandwich-based detection
Simplicity: Two steps processing
Efficiency: Get data within 4 hours
Multiplex: Simultaneous quantification of 5 biomarkers

Target Names

Scroll over each target protein for more information
Fas L Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6 Gene ID: 356 Accession #: P48023 ELISA Kit: NexaTag® Human FasL qIPCR ELISA Kit	IL-1 F10 Gene Symbol: IL1F10 FIL1T IL1HY2 IL38 FKSG75 UNQ6119/PRO20041 Gene ID: 84639 Accession #: Q8WWZ1 ELISA Kit: NexaTag® Human IL-1F10 qIPCR ELISA Kit	IL-24 Gene Symbol: IL24 MDA7 ST16 Gene ID: 11009 Accession #: Q13007 ELISA Kit: NexaTag® Human IL-24 qIPCR ELISA Kit	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: NexaTag® Human IL-3 qIPCR ELISA Kit	IL-33 Gene Symbol: IL33 C9orf26 IL1F11 NFHEV Gene ID: 90865 Accession #: O95760 ELISA Kit: NexaTag® Human IL-33 qIPCR ELISA Kit

Kit Components

Component	Size / Qty
96-well V-bottom Plate	1 plate
96-well Plate Film	1 piece
96-well PCR Plate*	1 plate
Assay Diluent	1 bottle
Wash Buffer	1 bottle
Lyophilized Standard Mix	1 vial
Antibody Coated Beads Mix	1 vial
Oligonucleotide Conjugated Antibody	1 vial
Elution Buffer	1 vial
PCR Master Mix	1 vial
Primer Mix	1 vial
TaqMan Probe Mix	1 vial
PCR Plate Film	1 piece

Additional Materials Required

Real-time PCR instrument capable of reading Real-time PCR instrument capable of detecting 5 channels in qPCR: FAM, VIC/HEX, ABY/TAMRA, JUN/ROX and Cy5 (Bio-Rad or QuantStudio™ recommended)
Orbital 96-well plate shaker (with ability to reach 700 rpm)
RayBio^® 96-well Magnetic Plate Separator (Catalog #: BIQ-MPS) or similar
Optional: Automated microplate washer (BioTek 405 TS Washer recommended) with magnetic separation (RayBio^® 96-well Magnetic Plate Separator recommended)
Precision pipettes to deliver 1 μL to 1 mL volumes
Pipette tips (barrier tips are recommended to reduce contamination)
Adjustable 1-25 mL pipettes for reagent preparation
Centrifuge that can spin 96 well plates at 500 g
Nuclease-free water or deionized water
1.5 mL, 2 mL, or 5 mL microcentrifuge tubes
Absorbent paper

Assay Procedure Summary

Prepare all reagents, samples and standards as instructed.
Add 5 µL of the bead and conjugate mix, and 5 µL of each standard and sample to each well. Incubate for 2.5 hours at room temperature while shaking.
Add 40 µL Assay Diluent and wash the plate five times using a magnetic plate separator or automated microplate washer.
Add 40 µL Elution buffer to each well. Incubate for 10 minutes at room temperature.
Prepare TaqMan Probe/Primer/PCR Master Mix and pipette 15 µL into each well of the PCR plate.
Transfer 5 µL supernatant from V-bottom plate to the PCR plate.
Run real-time PCR.

Featured Products

25% off all ELISA kits

25% off all ELISA kits

NexaTag^® Human Inflammation qIPCR Multiplex ELISA Array 4

Product Description

Specifications

Product Features

Target Names

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

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Product Description

Specifications

Product Features

Target Names

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

NexaTag® Human FasL qIPCR ELISA Kit

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NexaTag^® Human Inflammation qIPCR Multiplex ELISA Array 4

NexaTag® Human FasL qIPCR ELISA Kit

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NexaTag® Human IL-24 qIPCR ELISA Kit

NexaTag® Human IL-3 qIPCR ELISA Kit

NexaTag® Human IL-33 qIPCR ELISA Kit

NexaTag^® Human Inflammation qIPCR Multiplex ELISA Array 4

Featured Products

25% off all ELISA kits

25% off all ELISA kits

NexaTag® Human Inflammation qIPCR Multiplex ELISA Array 4

Product Description

Specifications

Product Features

Target Names

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

NexaTag® Human Inflammation qIPCR Multiplex ELISA Array 4NexaTag® Human Inflammation qIPCR Multiplex ELISA Array 4

Product Description

Specifications

Related Products

Product Features

Target Names

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Related Products

NexaTag® Human FasL qIPCR ELISA Kit

NexaTag® Human IL-1F10 qIPCR ELISA Kit

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NexaTag® Human Inflammation qIPCR Multiplex ELISA Array 4

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NexaTag^® Human Inflammation qIPCR Multiplex ELISA Array 4

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NexaTag^® Human Inflammation qIPCR Multiplex ELISA Array 4

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