Peroxidases, a group of heme-containing enzymes, catalyze oxidation-reduction reactions. They utilize hydrogen peroxide (H₂O₂) or lipid peroxides as substrates for various oxidative reactions. Peroxidases are present in bacteria, fungi, plants, and animals, and they play a crucial role in protecting cells from oxidative damage.

Peroxidase Activity Assay Kit offers a straightforward, reproducible, and highly sensitive method for measuring peroxidase activity in plasma, serum, cell lysates, urine, and other biological liquid samples without requiring any pre-treatment. In this assay, an ultra-sensitive colorimetric probe reacts with both hydrogen peroxide (H₂O₂) and peroxidase enzyme to generate a pink-colored product. The intensity of the pink color is then quantified at 570 nm, providing a direct measure of the peroxidase content in the sample.

• Microplate reader capable of measuring absorbance at 570 nm
• Precision pipettes to deliver 2 µL to 1 mL volumes
• Multi-channel pipettes to deliver 20 μL to 200 μL volumes
• Tubes to prepare sample dilutions
• 15 mL conical tubes

Each peroxidase standard and sample should be assayed in duplicate or triplicate. A freshly prepared standard curve should be used each time the assay is performed.

1. Add 50 µL of the diluted Horseradish Peroxidase standards or unknown samples to each well of the Microplate.
2. Add 50 µL of the Chromogenic Working Solution to each well. Incubate the plate at room temperature for 30 minutes gently mixing in the dark.
3. Measure the absorbance at 570nm using a plate reader.