Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

The RayBiotech Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit measures phosphorylated Erk1 (Thr202/Tyr204) + Erk2 (Thr185/Tyr187) and total (pan) Erk1 + 2 in cell and tissue lysates.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human, Mouse, Rat
Specificity	The antibody pair provided in this kit recognizes human, mouse and rat Phospho-Erk1 (pThr202/pTyr204) + Erk2 (pTyr185/187).
Compatible Sample Types	Tissue Lysates, Cell Lysates
Accession Number	P27361, P28482
Gene Id	5595, 5594
Gene Symbols	MAPK1, MAPK3, ERK1, PRKM3, ERK2, PRKM1, PRKM2
Protein Name / Synonyms	Mitogen-activated protein kinase 3 (MAP kinase 3) (MAPK 3) (EC 2.7.11.24) (ERT2) (Extracellular signal-regulated kinase 1) (ERK-1) (Insulin-stimulated MAP2 kinase) (MAP kinase isoform p44) (p44-MAPK) (Microtubule-associated protein 2 kinase) (p44-ERK1) Mitogen-activated protein kinase 1 (MAP kinase 1) (MAPK 1) (EC 2.7.11.24) (ERT1) (Extracellular signal-regulated kinase 2) (ERK-2) (MAP kinase isoform p42) (p42-MAPK) (Mitogen-activated protein kinase 2) (MAP kinase 2) (MAPK 2)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Post-Translational Modifications, Phosphorylation, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Simultaneously measure phosphorylated protein and pan protein in one experiment (for normalization purpose)
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Pre-Coated 96-well Strip Microplate
Positive Control Sample
Anti-Phospho Antibody
Anti-Pan Antibody (*not included in phospho-only version)
HRP-Conjugated Secondary Antibody
Wash Buffer
Assay Diluent
Lysis Buffer
TMB One-Step Substrate
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Shaker
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Tubes to prepare sample dilutions

Additional Materials Required

Prepare all reagents, samples and positive control as instructed in the manual.
Add 100 μL of positive control or sample to each well.
Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 μL prepared detection antibody to each well.
Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL prepared HRP-Conjugated solution.
Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL TMB One-Step Substrate Reagent to each well.
Incubate 30 minutes at room temperature.
Add 50 μL Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (PEL-Erk-T202-T.pdf)

Datasheet (PEL-Erk-T202.pdf)

SDS (9eba300983fca9631bcc6e7a5a880144820e9f01.pdf)

SDS (32c7f69976571d2f7e0931fa88352f97bb49b054.pdf)

Product Features

Simultaneously measure phosphorylated protein and pan protein in one experiment (for normalization purpose)
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Pre-Coated 96-well Strip Microplate
Positive Control Sample
Anti-Phospho Antibody
Anti-Pan Antibody (*not included in phospho-only version)
HRP-Conjugated Secondary Antibody
Wash Buffer
Assay Diluent
Lysis Buffer
TMB One-Step Substrate
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Shaker
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Tubes to prepare sample dilutions

Additional Materials Required

Prepare all reagents, samples and positive control as instructed in the manual.
Add 100 μL of positive control or sample to each well.
Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 μL prepared detection antibody to each well.
Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL prepared HRP-Conjugated solution.
Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL TMB One-Step Substrate Reagent to each well.
Incubate 30 minutes at room temperature.
Add 50 μL Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Additional Materials Required

Storage/Stability

Have this product in hand?

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Additional Materials Required

Storage/Stability

Have this product in hand?

Works Great!

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Works Great!

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Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Additional Materials Required

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

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Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Additional Materials Required

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Works Great!

Ask A Question

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Phospho-Erk1 (T202/Y204) + Erk2 (T185/Y187) ELISA Kit

Works Great!

Services

Resources

About Us

Contact Us

Featured Products