Phospho-p38 alpha (T180/Y182) ELISA Kit

The RayBiotech Phospho-p38 alpha (T180/Y182) ELISA Kit measures phosphorylated p38 alpha (Thr180/Tyr182) and total (pan) p38 alpha in cell and tissue lysates.

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human, Mouse, Rat
Specificity	The antibody pair provided in this kit recognizes human and mouse Phospho-P38 alpha (pThr180/pTyr182).
Compatible Sample Types	Tissue Lysates, Cell Lysates
Accession Number	Q16539
Gene Id	1432
Gene Symbols	MAPK14, CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A
Protein Name / Synonyms	Mitogen-activated protein kinase 14 (MAP kinase 14) (MAPK 14) (EC 2.7.11.24) (Cytokine suppressive anti-inflammatory drug-binding protein) (CSAID-binding protein) (CSBP) (MAP kinase MXI2) (MAX-interacting protein 2) (Mitogen-activated protein kinase p38 alpha) (MAP kinase p38 alpha) (Stress-activated protein kinase 2a) (SAPK2a)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Sandwich-based
Method Of Detection	Colorimetric
Research Area	Angiogenesis, Cancer, Post-Translational Modifications, Inflammation, Phosphorylation, MAPK Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Simultaneously measure phosphorylated protein and pan protein in one experiment (for normalization purpose)
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Pre-Coated 96-Well Microplate
Positive Control
Anti-Phospho Protein Antibody
Anti-Pan Protein Antibody (*not included in phospho-only version)
HRP-Conjugated Secondary Antibody
Wash Buffer
Assay Diluent
Lysis Buffer
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Shaker
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Tubes to prepare the positive control or sample dilutions

Assay Procedure Summary

Prepare all reagents, samples and positive control as instructed in the manual.
Add 100 μL of positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Have this product in hand?

Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (PEL-P38-T180-T.pdf)

Datasheet (PEL-P38-T180.pdf)

SDS (703c5446da2ab4a2e56c90c0fde539aef94aa4aa.pdf)

SDS (fba1649d6921bcf9f5037ed717f125fba2aeaa11.pdf)

Product Features

Simultaneously measure phosphorylated protein and pan protein in one experiment (for normalization purpose)
Screen numerous different cell lysates without performing a Western Blot analysis
Minimal hands-on time, convenient, and non-radioactive material

Kit Components

Pre-Coated 96-Well Microplate
Positive Control
Anti-Phospho Protein Antibody
Anti-Pan Protein Antibody (*not included in phospho-only version)
HRP-Conjugated Secondary Antibody
Wash Buffer
Assay Diluent
Lysis Buffer
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

Microplate reader capable of measuring absorbance at 450 nm
Protease and Phosphatase inhibitors
Shaker
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 ml pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Log-log graph paper or computer and software for ELISA data analysis
Tubes to prepare the positive control or sample dilutions

Assay Procedure Summary

Prepare all reagents, samples and positive control as instructed in the manual.
Add 100 μL of positive control or sample to each well. Incubate 2.5 hours at room temperature or overnight at 4°C with gentle shaking.
Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL prepared HRP-Conjugated solution. Incubate for 1 hour at room temperature with gentle shaking.
Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
Add 50 μL Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Featured Products

Phospho-p38 alpha (T180/Y182) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Phospho-p38 alpha (T180/Y182) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Human/Mouse/Rat Phospho-p38 MAPK (T180/Y182) Cell-Based ELISA Kit

Anti-MAPK14 (T180/Y182) Antibody

Anti-Phospho-IKKB (T180) Antibody

Recombinant Human Mitogen-Activated Protein Kinase 14/MAPK 14, Antigen Grade

Phospho-p38 alpha (T180/Y182) ELISA Kit

Phospho-p38 alpha (T180/Y182) ELISA Kit

Human/Mouse/Rat Phospho-p38 MAPK (T180/Y182) Cell-Based ELISA Kit

Anti-MAPK14 (T180/Y182) Antibody

Anti-Phospho-IKKB (T180) Antibody

Recombinant Human Mitogen-Activated Protein Kinase 14/MAPK 14, Antigen Grade

Featured Products

Phospho-p38 alpha (T180/Y182) ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Phospho-p38 alpha (T180/Y182) ELISA Kit

Product Description

Specifications

Related Products

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Related Products

Human/Mouse/Rat Phospho-p38 MAPK (T180/Y182) Cell-Based ELISA Kit

Anti-MAPK14 (T180/Y182) Antibody

Anti-Phospho-IKKB (T180) Antibody

Recombinant Human Mitogen-Activated Protein Kinase 14/MAPK 14, Antigen Grade

Phospho-p38 alpha (T180/Y182) ELISA Kit

Phospho-p38 alpha (T180/Y182) ELISA Kit

Human/Mouse/Rat Phospho-p38 MAPK (T180/Y182) Cell-Based ELISA Kit

Anti-MAPK14 (T180/Y182) Antibody

Anti-Phospho-IKKB (T180) Antibody

Recombinant Human Mitogen-Activated Protein Kinase 14/MAPK 14, Antigen Grade