Porcine IL-1 beta ELISA

$398.00
ELP-IL1b-1

RayBio® Porcine (pig) IL-1 beta (IL-1 F2) ELISA Kit for cell culture supernatants, plasma, and serum samples.

Product Features

Sensitivity
6 pg/ml

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Detection Range
6 pg/ml - 6000 pg/ml
Recommended Dilution (serum & plasma)
2 fold
Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Specificity

The sandwich ELISA antibody pair detects Porcine IL-1 beta.
Species Detected
Porcine (pig)
Protein Name & Synonyms
Interleukin-1 beta (IL-1 beta)
Accession Number
Gene Names
IL1B
Gene ID
397122

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Antigen Information

Gene Symbol:
  • IL1B

Product Specifications

Size:
1, 2, or 5 x 96-Well Strip Microplate Kit

Array/ELISA Format

Species Detected:
  • Porcine (pig)
Solid Support:
96-well Microplate
Design Principle:
  • Sandwich-based
Quantitative/Semi-Quantitative:
Quantitative
Method of Detection:
Colorimetric
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants

  1. Zong X., Song D., Wang T., et al. LFP-20, a porcine lactoferrin peptide, ameliorates LPS-induced inflammation via the MyD88/NF-κ and MyD88/MAPK signaling pathways. Dev Comp Immunol. 2015 Oct;52(2):123-31. doi: 10.1016/j.dci.2015.05.006.
    Species: Porcine
    Sample Type: Conditioned Media

  2. Li J., Hu L., Liu Y., et al. DDX19A Senses Viral RNA and Mediates NLRP3-Dependent Inflammasome Activation. J Immunol. 2015 Dec 15;195(12):5732-49. doi: 10.4049/jimmunol.1501606
    Species: Porcine
    Sample Type: Conditioned Media

  3. Pomorska-Mol M., et al. Local and systemic immune response in pigs during subclinical and clinical swine influenza infection. Research in Veterinary Sciences, June 2014 epub ahead of print. http://dx.doi.org/10.1016/j.rvsc.2014.06.007
    Species: Porcine
    Sample Type: Tissue Lysate

  4. Pomorska-Mol., Markowska-Daniel I., Kwit K., Czyzewska E., et al. Immune and inflammatory response in pigs during acute influenza caused by H1N1 swine influenza virus. Archives of Virology, 2014 May Epub Ahead of Print. DOI:10.1007/s00705-014-2116-1
    Species: Porcine
    Sample Type: Tissue Lysate

  5. Zhang K, Hou Q, Zhong Z, et al. Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E. Virology. 2013 Aug 1;442(2):156-162.
    Species: Porcine
    Sample Type: Conditioned Media

  6. Galindo R., Ayllon N., Smrdel K., et al. Gene expression profile suggests that pigs (Sus scrofa) are susceptible to Anaplasma phagocytophilum but control infection. Parasit Vectors. 2012 Aug 30;5:181.
    Species: Porcine
    Sample Type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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