Porcine IL-6 ELISA

RayBio® Porcine (pig) IL-6 ELISA Kit for cell culture supernatants, plasma, and serum samples.


Antigen Information

Accession Number:
  • P26893
Gene ID:
  • 399500
  • 100628202
Gene Symbols:
  • IL6
Protein Name & Synonyms:
Interleukin-6 (IL-6)
Target Species:
  • Porcine (pig)

Assay Format

Detection Range:
45 pg/ml - 10000 pg/ml
Recommended Dilution (serum & plasma):
2 fold
45 pg/ml

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The sandwich ELISA antibody pair detects Porcine IL-6.
Number of Targets Detected:
Species Detected:
  • Porcine (pig)
Compatible Sample Types:
  • Cell Culture Supernatants
  • Plasma
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Solid Support:
96-well Microplate

Product Specifications

1, 2, or 5 x 96-Well Strip Microplate Kit

Product Features

Additional Notes
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data

Standard Curves

Spiking & Recovery Results

Linearity Results

Application Notes

Kit Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Other Materials Required
  • Distilled or deionized water
  • Precision pipettes to deliver 2 µl to 1 µl volumes
  • Adjustable 1-25 µl pipettes for reagent preparation
  • 100 µl and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 µl of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 µl of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 µl of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 µl of Stop Solution to each well.
  11. Read at 450 nm immediately.


The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Zong X., Song D., Wang T., et al. LFP-20, a porcine lactoferrin peptide, ameliorates LPS-induced inflammation via the MyD88/NF-?B and MyD88/MAPK signaling pathways. Dev Comp Immunol. 2015 Oct;52(2):123-31. doi: 10.1016/j.dci.2015.05.006.
    Species: Porcine
    Sample type: Conditioned Media
  2. Dorcaratto D, Burdío F, Fondevila D, et al. Radiofrequency is a secure and effective method for pancreatic transection in laparoscopic distal pancreatectomy: results of a randomized, controlled trial in an experimental model. Surg Endosc. 2013 Apr 13; doi:10.1007/s00464-013-2952-1 [Epub ahead of print]
    Species: Porcine
    Sample type: Peritoneal Fluid
  3. Tomosada Y, Villena J, Murata K, et al. Immunoregulatory effect of bifidobacteria strains in porcine intestinal epithelial cells through modulation of ubiquitin-editing enzyme A20 expression. PLoS ONE. 2013;8(3):e59259.
    Species: Porcine
    Sample type: Conditioned Media
  4. Zhang K, Hou Q, Zhong Z, et al. Porcine reproductive and respiratory syndrome virus activates inflammasomes of porcine alveolar macrophages via its small envelope protein E. Virology. 2013 Aug 1;442(2):156-162.
    Species: Porcine
    Sample type: Conditioned Media
  5. Li, J., and I. H. Kim. "Effects of levan-type fructan supplementation on growth performance, digestibility, blood profile, fecal microbiota, and immune responses after lipopolysaccharide challenge in growing pigs." Journal of animal science 91.11 (2013): 5336-5343.
    Species: Porcine
    Sample type: Serum
  6. Shimazu T., et al. Immunobiotic Lactobacillus jensenii Elicits Anti-Inflammatory Activity in Porcine Intestinal Epithelial Cells by Modulating Negative Regulators of the Toll-Like Receptor Signaling Pathway. Infect. Immun. 2012, 80(1):276. DOI: 10.1128/IAI.05729-11.
    Species: Porcine
    Sample type: Conditioned Media
  7. Shimazu T., Villena J., Tohno M., et al Immunobiotic Lactobacillus jensenii elicits anti-inflammatory activity in porcine intestinal epithelial cells by modulating negative regulators of the Toll-like receptor signaling pathway. Infect Immun. 2012 Jan;80(1):276-88. doi: 10.1128/IAI.05729-11.
    Species: Porcine
    Sample type: Conditioned Media
  8. Emaminia, Abbas, et al. "Adenosine A 2A agonist improves lung function during ex vivo lung perfusion." The Annals of thoracic surgery 92.5 (2011): 1840-1846.
    Species: Pig
    Sample type: Tissue Lysate

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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