Phospho-BCAR1-Y165 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P56945 |
| Gene Id | 9564 |
| Gene Symbols | BCAR1 |
| Protein Name / Synonyms | BCAR1, CAS, CAS1, CASS1, CRKAS, P130Cas, BCAR1, Cas family scaffolding protein |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
77kDa/93kDa/95kDa/97kDa
Observed MW
129kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding Y165 of human BCAR1
Modification
Phospho Y165
Storage
-20°C
Images
Western blot analysis of extracts of PC-12 cells, using Phospho-BCAR1-Y165 antibody (144-61532) at 1:1000 dilution. PC-12 cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 90s.
Expiration:
12 months from the date of shipment when stored properly.