CHUK Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O15111 |
| Gene Id | 1147 |
| Gene Symbols | CHUK |
| Protein Name / Synonyms | CHUK, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16, conserved helix-loop-helix ubiquitous kinase |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF, IP |
| Research Area | Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
Format
Calculated MW
84kDa
Observed MW
94kDa
Immunogen
A synthetic peptide of human CHUK
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using CHUK antibody (144-02062) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded rat brain using CHUK antibody (144-02062) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CHUK antibody (144-02062) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using CHUK antibody (144-02062). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of 293T cells using 3ug CHUK antibody (144-02062). Western blot was performed from the immunoprecipitate using CHUK antibody (144-02062) at a dilition of 1:1000.
Expiration:
12 months from the date of shipment when stored properly.