Phospho-CRKL-Y207 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P46109 |
| Gene Id | 1399 |
| Gene Symbols | CRKL |
| Protein Name / Synonyms | CRKL, crk-like protein |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a protein kinase containing SH2 and SH3 (src homology) domains which has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase, plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic.
Dilutions
WB 1:500 - 1:2000
IHC 1:50 - 1:100
IHC 1:50 - 1:100
Format
Calculated MW
33kDa
Observed MW
38kDa
Immunogen
A synthetic phosphorylated peptide around Y207 of human CRKL (NP_005198.1).
Modification
Phospho Y207
Storage
-20°C
Images
Western blot analysis of extracts of K-562 cells, using Phospho-CRKL-Y207 antibody (144-61538) at 1:1000 dilution or CRKL antibody (144-11735). K-562 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 5s.
Expiration:
12 months from the date of shipment when stored properly.