Phospho-CTNNB1-S675 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | P35222 |
| Gene Id | 1499 |
| Gene Symbols | CTNNB1 |
| Protein Name / Synonyms | CTNNB1, CTNNB, MRD19, armadillo, catenin beta-1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis, Cardiovascular Disease, Cancer, Wnt-beta-Catenin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
9kDa/85kDa
Observed MW
100kDa
Immunogen
A synthetic phosphorylated peptide around S675 of human CTNNB1 (NP_001091679.1).
Modification
Phospho S675
Storage
-20°C
Images
Western blot analysis of extracts of C6 cells, using Phospho-CTNNB1-S675 antibody (144-61524) at 1:1000 dilution. C6 cell lysates were treated by CIP at 37°C for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 180s.
Expiration:
12 months from the date of shipment when stored properly.