Phospho-DPYSL2-T514 pAb
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q16555 |
| Gene Id | 1808 |
| Gene Symbols | DPYSL2 |
| Protein Name / Synonyms | DPYSL2, CRMP-2, CRMP2, DHPRP2, DRP-2, DRP2, N2A3, ULIP-2, ULIP2, dihydropyrimidinase like 2 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a member of the collapsin response mediator protein family. Collapsin response mediator proteins form homo- and hetero-tetramers and facilitate neuron guidance, growth and polarity. The encoded protein promotes microtubule assembly and is required for Sema3A-mediated growth cone collapse, and also plays a role in synaptic signaling through interactions with calcium channels. This gene has been implicated in multiple neurological disorders, and hyperphosphorylation of the encoded protein may play a key role in the development of Alzheimer's disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
58kDa/62kDa
Observed MW
69kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T514 of human DPYSL2
Modification
Phospho T514
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using Phospho-DPYSL2-T514 antibody (144-61537) at 1:1000 dilution or DPYSL2 antibody (144-60712). SH-SY5Y cells were treated by CIP(20uL/400ul) at 37? for 1 hour. PC-12 cells were treated by CIP(20uL/400ul) at 37? for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 1s.
Expiration:
12 months from the date of shipment when stored properly.