Phospho-EEF2K-S366 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | O00418 |
| Gene Id | 29904 |
| Gene Symbols | EEF2K |
| Protein Name / Synonyms | EEF2K, HSU93850, eEF-2K, eukaryotic elongation factor 2 kinase |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes a highly conserved protein kinase in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of this kinase is increased in many cancers and may be a valid target for anti-cancer treatment.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
82kDa
Observed MW
105kDa
Immunogen
A synthetic phosphorylated peptide around S366 of human EEF2K (NP_037434.1).
Modification
Phospho S366
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-EEF2K-S366 antibody (144-61550) at 1:1000 dilution. Hela cell lysate were treated by CIP (20ul CIP for each 400ul cell lysate) at 37? for 1 hour or treated by PMA/TPA (200nM) for 15 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 15s.
Expiration:
12 months from the date of shipment when stored properly.