Phospho-EIF4EBP1-T70 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13541 |
| Gene Id | 1978 |
| Gene Symbols | EIF4EBP1 |
| Protein Name / Synonyms | EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, eukaryotic translation initiation factor 4E-binding protein 1 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | mTOR Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E), which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of mRNA translation.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
12kDa
Observed MW
17kDa
Immunogen
A phospho specific peptide corresponding to residues surrounding T70 of human EIF4EBP1
Modification
Phospho T70
Storage
-20°C
Images
Western blot analysis of extracts of HeLa cells, using Phospho-EIF4EBP1-T70 antibody (144-61487) at 1:2000 dilution. HeLa cells were treated by Insulin (100 nM) at 37? for 5 minutes after serum-starvation overnight.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit.
Exposure time: 3min.
Expiration:
12 months from the date of shipment when stored properly.