Phospho-EphA2-Y594 pAb
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Rat |
| Host Species | Rabbit |
| Accession Number | P29317 |
| Gene Id | 1969 |
| Gene Symbols | EPHA2 |
| Protein Name / Synonyms | EPHA2, ARCC2, CTPA, CTPP1, CTRCT6, ECK, EPH receptor A2 |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting |
| Research Area | Angiogenesis, Cancer, Inflammation |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. This gene encodes a protein that binds ephrin-A ligands. Mutations in this gene are the cause of certain genetically-related cataract disorders.
Dilutions
WB 1:500 - 1:2000
Format
Calculated MW
54kDa/108kDa
Observed MW
90kDa
Immunogen
A synthetic phosphorylated peptide around Y594 of human EphA2 (NP_004422.2).
Modification
Phospho Y594
Storage
-20°C
Images
Western blot analysis of extracts of SH-SY5Y cells, using Phospho-EphA2-Y594 antibody (144-61534) at 1:1000 dilution. SH-SY5Y cells were treated by EphrinA1 (100 ng/ml) for 5 minutes .
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit.
Exposure time: 60s.
Expiration:
12 months from the date of shipment when stored properly.