FKBP5 Polyclonal Antibody
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Product Description
Specifications
| Size | 20 µL, 100 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse, Rat |
| Host Species | Rabbit |
| Accession Number | Q13451 |
| Gene Id | 2289 |
| Gene Symbols | FKBP5 |
| Protein Name / Synonyms | FKBP5, AIG6, FKBP51, FKBP54, P54, PPIase, Ptg-10, FK506 binding protein 5 |
| Clonality | Polyclonal |
| Isotype | IgG |
| Purity | Affinity purification |
| Buffers | PBS with 0.02% sodium azide, 50% glycerol, pH7.3 |
| Recommended Applications | Western Blotting, IHC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Background
The protein encoded by this gene is a member of the immunophilin protein family, which play a role in immunoregulation and basic cellular processes involving protein folding and trafficking. This encoded protein is a cis-trans prolyl isomerase that binds to the immunosuppressants FK506 and rapamycin. It is thought to mediate calcineurin inhibition. It also interacts functionally with mature hetero-oligomeric progesterone receptor complexes along with the 90 kDa heat shock protein and P23 protein. This gene has been found to have multiple polyadenylation sites. Alternative splicing results in multiple transcript variants.
Dilutions
WB 1:200 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IHC 1:50 - 1:200
IF 1:50 - 1:200
Format
Calculated MW
30kDa/51kDa
Observed MW
51kDa
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 308-457 of human FKBP5 (NP_001139247.1).
Modification
Unmodified
Storage
-20°C
Images
Western blot analysis of extracts of various cell lines, using FKBP5 antibody (144-61373) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human colon using FKBP5 antibody (144-61373) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using FKBP5 antibody (144-61373) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using FKBP5 antibody (144-61373) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using FKBP5 antibody (144-61373) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using FKBP5 antibody (144-61373) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using FKBP5 antibody (144-61373) at dilution of 1:100. Blue: DAPI for nuclear staining.
Expiration:
12 months from the date of shipment when stored properly.