KLH conjugated synthetic peptide (CDYKDDDDKDYKDDDDK) was used as antigen.
Epitope tags are useful for the labeling and detection of recombinant proteins using western blotting, immunoprecipitation and immunostaining techniques. The eight amino acid DYKDDDDK peptide is an established and multi-functional epitope tag and can be expressed and detected with a recombinant protein as an amino-terminal or carboxy-terminal fusion (1). RayBiotech's DYKDDDDK antibody binds to the same epitope recognized by Sigma's Anti-FLAG antibodies. (FLAG is a registered trademark of Sigma-Aldrich Co., which is not affiliated with RayBiotech).
Tag from influenza hemagglutinin protein
Purified Rabbit Polyclonal Antibody (Pab)
Western blot analysis of lysate from 12tag protein, using FLAG tag antibody (DYKDDDDK)(Cat. #102-10563). 102-10563 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.
2-8°C (short-term); -20°C (long-term)
12 months from the date of shipment when stored properly.