Rabbit Anti-FOXG1 (Center) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human, Mouse |
| Host Species | Rabbit |
| Accession Number | P55316 |
| Gene Id | 2290 |
| Gene Symbols | FOXG1 |
| Protein Name / Synonyms | FOXG1 (Center) |
| Clonality | Polyclonal |
| Preparation | This FOXG1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-252 amino acids from the Central region of human FOXG1. |
| Concentration (lot specific) | 0.5 mg/ml |
| Recommended Applications | Western Blotting, FC, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
This gene belongs to the forkhead family of transcription factors which is characterized by a distinct forkhead domain. The specific function of this gene has not yet been determined; however, it may play a role in the development of the brain and telencephalon.
Specificity
Predicted Reactivity
Rat, Chicken, Drosophila, Xenopus
Other Names
FOXG1; FKH2; FKHL1; FKHL2; FKHL3; FKHL4; FOXG1A; FOXG1B; FOXG1C; Forkhead box protein G1; Brain factor 1; Brain factor 2; Forkhead box protein G1A; Forkhead box protein G1B; Forkhead box protein G1C; Forkhead-related protein FKHL1; Forkhead-related protein FKHL2; Forkhead-related protein FKHL3
NCBI Accession #
NP_005240.3
Other Accession #
P32031; Q9YHC5; Q00939; Q60987; Q90964
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
52352
Recommended Dilutions
WB: 1:1000; FC: 1:10-50; IF: 1:10-50
 | Western blot analysis of FOXG1 Antibody (Center) (Cat# 102-18396) in mouse brain tissue lysates (35ug/lane). FOXG1 (arrow) was detected using the purified Pab. |
 | FOXG1 Antibody (Center) (Cat# 102-18396) flow cytometric analysis of U251 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Fluorescent confocal image of A549 cell stained with FOXG1 Antibody (Center)(Cat# 102-18396).A549 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with FOXG1 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). FOXG1 immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.