Synonyms
C17orf25 antibody, HC71 antibody, GLOD4 antibody, glyoxalase domain-containing protein 4 antibody, glyoxalase domain containing 4 antibody
Specificity
Species Reactivity
Human, Mouse
Format
Product Type
Primary antibody
Formulation
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Calculated Molecular Weight
35 kDa
Immunogen
Recombinant protein fragment contain a sequence corresponding to a region within amino acids 5 and 217 of GLOD4
Applications
Applications Tested
IHC (Formalin-fixed paraffin-embedded sections),Western blot
Application Notes
Suggested starting dilutions are as follows: WB: 1:500-1:3000, IHC-P: 1:100-1:1000 [suggested antigen retrieval using heat mediated 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0)]. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.

Immunohistochemical analysis of paraffin-embedded gastric , using GLOD4(107-10455) antibody at 1:100 dilution.

Sample (50 ug of whole cell lysate)
A: Mouse brain
12% SDS PAGE
107-10455 diluted at 1:1000

Sample(30 µg of whole cell lysate)
A:Hep G2
12% SDS PAGE
107-10455 diluted at 1:1000
Storage
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Expiration:
12 months from the date of shipment when stored properly.